The multigene family of fungal laccases and their expression in the white rot basidiomycete Flammulina velutipes

Gene
Wei WangBao-Gui Xie

Abstract

Fungal laccases play important roles in matrix degradation. Eleven laccase genes, including three novel ones (designated lac1, lac2 and lac4) were identified after sequencing the entire genome of the edible, white-rot fungus Flammulina velutipes. Analysis using bioinformatics revealed that all of the laccases, except lac3, possess a signal peptide. These laccase proteins consist of 502-670 amino acids and have predicted molecular weights ranging from 55kDa to 74kDa. These proteins each contain four copper-binding sites, except for Lac10. Transcriptomes were sequenced at different developmental stages and in different fruiting body tissues to analyze if there was differential expression of laccase genes. The novel laccase gene lac4 exhibited the highest expression levels among all of the observed laccases at every developmental stage and in all fruiting body tissues examined. We conclude that laccases in F. velutipes play a role not only in lignin degradation, but also in fruiting body formation and development.

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Citations

Dec 4, 2015·International Journal of Molecular Sciences·Yuanping LuBaogui Xie
Aug 17, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jie YangXiuyun Ye
Dec 6, 2016·International Journal of Molecular Sciences·Yuan-Ping LuBao-Gui Xie
Feb 9, 2018·International Journal of Molecular Sciences·Wensong JinZonghua Wang
Apr 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoyu JiaoYongping Cai
Feb 7, 2020·BioMed Research International·Yuan-Ping LuMei-Yuan Chen
May 2, 2020·Applied Microbiology and Biotechnology·Taju WuShaojie Li
Jun 2, 2017·Journal of Agricultural and Food Chemistry·Jing-Yu LiuMing-Liang Zhang

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