PMID: 8954164Nov 15, 1996Paper

The multiple forms of starch-branching enzyme I in Solanum tuberosum

European Journal of Biochemistry
J KhoshnoodiH Larsson

Abstract

Western blot analysis showed the presence of three forms of starch-branching enzyme (SBE), with apparent molecular masses of 103, 97 and 80 kDa, in extracts of leaves and stored tubers of Solanum tuberosum. The 80-kDa form was absent in extracts of fresh tuber. Active 80-kDa enzyme was partially purified from stored tubers and sequence analysis showed that it, similar to the two larger enzyme forms, was an SBE-I isoform. Limited proteolysis of isolated 103-kDa SBE-I under native conditions removed approximately 200 amino acid residues from the carboxy terminus. A stable intermediate with an apparent molecular mass of approximately 80 kDa was formed. Since the 80-kDa form displayed full enzymatic activity and its circular-dichroism spectrum did not differ significantly from that of the 103-kDa enzyme, the carboxy-terminal portion of the enzyme was suggested to have an extended, unordered structure and therefore to be easily accessible to proteolysis. A cDNA sequence encoding a mature SBE-I was amplified from tuber mRNA of S. tuberosum by means of PCR. The 3' end of this sequence differed significantly from that of previously published data. PCR amplification and DNA sequencing of the 3' ends of the sbeI gene showed that four sbe...Continue Reading

References

Nov 1, 1992·Journal of Biochemistry·K MizunoT Baba
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May 15, 1987·Analytical Biochemistry·J LogemannL Willmitzer
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Citations

Dec 6, 2001·European Journal of Biochemistry·U RydbergH Larsson
Jan 7, 2017·BMC Genomics·Jessica K Van HarsselaarSophia Sonnewald
Sep 9, 2010·Journal of Agricultural and Food Chemistry·Jia-Wei ChangYuan-Tih Ko

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