The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineages.

The Plant Journal : for Cell and Molecular Biology
Takao KoedukaEran Pichersky

Abstract

Many plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Clarkia breweri flowers emit a mixture of eugenol and isoeugenol, while Petunia hybrida flowers emit mostly isoeugenol with small amounts of eugenol. We recently reported the identification of a petunia enzyme, isoeugenol synthase 1 (PhIGS1) that catalyzes the formation of isoeugenol, and an Ocimum basilicum (basil) enzyme, eugenol synthase 1 (ObEGS1), that produces eugenol. ObEGS1 and PhIGS1 both utilize coniferyl acetate, are 52% sequence identical, and belong to a family of NADPH-dependent reductases involved in secondary metabolism. Here we show that C. breweri flowers have two closely related proteins (96% identity), CbIGS1 and CbEGS1, that are similar to ObEGS1 (58% and 59% identity, respectively) and catalyze the formation of isoeugenol and eugenol, respectively. In vitro mutagenesis experiments demonstrate that substitution of only a single residue can substantially affect the product specificity of these enzymes. A third C. breweri enzyme identified, CbEGS2, also catalyzes the formation of eugenol from coniferyl acetate and is only 46% identical to CbIGS1 an...Continue Reading

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Citations

Nov 13, 2008·Plant Molecular Biology·Masataka KajikawaTakashi Hashimoto
Nov 12, 2010·Journal of Experimental Botany·Thomas A ColquhounDavid G Clark
May 17, 2013·Plant & Cell Physiology·Shubhra RastogiAjit K Shasany
Jul 8, 2010·Plant Physiology·Geng YuEran Pichersky
Feb 1, 2011·Annual Review of Plant Biology·Eran Pichersky, Efraim Lewinsohn
Sep 23, 2014·Functional & Integrative Genomics·Alok K GuptaFlorian P Schiestl
Jun 22, 2011·Metabolic Engineering·Thomas HoffmannWilfried Schwab
Nov 6, 2009·Phytochemistry·Thomas A ColquhounDavid G Clark
Feb 7, 2013·The New Phytologist·Natalia DudarevaIan Kaplan
Jul 6, 2014·The New Phytologist·Joëlle K MuhlemannNatalia Dudareva
Mar 5, 2014·Plant, Cell & Environment·Joëlle K MuhlemannNatalia Dudareva
Oct 9, 2009·The Plant Journal : for Cell and Molecular Biology·Thomas A ColquhounDavid G Clark
Apr 24, 2015·The Plant Journal : for Cell and Molecular Biology·Yar-Khing YaukRoss G Atkinson
Nov 18, 2014·Plant, Cell & Environment·Alon Cna'aniAlexander Vainstein
Apr 3, 2013·Journal of Proteomics·Irene AragüezVictoriano Valpuesta
May 28, 2013·Biochemical and Biophysical Research Communications·Takao KoedukaJun Hiratake
Mar 7, 2017·The New Phytologist·Jasmin RavidAlexander Vainstein
Apr 6, 2017·The Plant Journal : for Cell and Molecular Biology·Yar-Khing YaukRoss G Atkinson
Jan 18, 2018·Bioscience, Biotechnology, and Biochemistry·Takao Koeduka
Aug 21, 2019·Frontiers in Plant Science·Keiko Yonekura-SakakibaraRyo Nakabayashi
Apr 23, 2020·Biotechnology for Biofuels·Chien-Yuan Lin, Aymerick Eudes
Dec 2, 2020·International Journal of Molecular Sciences·Jesús Picazo-AragonésFrancisco Balao
Jan 16, 2021·International Journal of Molecular Sciences·Vaishnavi Amarr ReddySarojam Rajani
Feb 20, 2021·Journal of Experimental Botany·Ryan M PatrickYing Li
May 2, 2015·Plant Physiology·Laura Medina-PucheJuan Muñoz-Blanco
Jun 9, 2021·Biochemical Society Transactions·Christopher J RobinsonNigel S Scrutton
Jan 12, 2019·Journal of Agricultural and Food Chemistry·Pauline RousserieLaurence Geny-Denis
Dec 23, 2016·Journal of Agricultural and Food Chemistry·Ross G Atkinson

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