The multistress-induced Translocator protein (TSPO) differentially modulates storage lipids metabolism in seeds and seedlings

The Plant Journal : for Cell and Molecular Biology
Pawel JurkiewiczHenri Batoko

Abstract

Translocator proteins (TSPO) are conserved membrane proteins extensively studied in mammals, but their function is still unclear. Angiosperm TSPO are transiently induced by abiotic stresses in vegetative tissues. We showed previously that constitutive expression of the Arabidopsis TSPO (AtTSPO) could be detrimental to the cell. Degradation of AtTSPO requires an active autophagy pathway. We show here that genetic modifications of TSPO expression in plant and yeast cells reduce the levels of cytoplasmic lipid droplets (LD). Transgenic Arabidopsis seedlings overexpressing AtTSPO contain less LD as compared with wild type (WT). LD levels were increased in Arabidopsis AtTSPO knockout (KO) seedlings. Deletion of the Schizosaccharomyces pombe TSPO resulted in an increase in LD level in the cell. As compared with the WT, the mutant strain was more sensitive to cerulenin, an inhibitor of fatty acids and sterol biosynthesis. We found that in contrast with seedlings, overexpression of AtTSPO (OE) resulted in an up to 50% increase in seeds fatty acids as compared with WT. A time course experiment revealed that after 4 days of seed imbibition, the levels of triacylglycerol (TAG) was still higher in the OE seeds as compared with WT or KO see...Continue Reading

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Citations

Mar 21, 2020·Frontiers in Plant Science·Brandon CalifarRobert J Ferl
Mar 2, 2019·Progress in Lipid Research·Thomas VanherckeSurinder P Singh
Jul 1, 2021·Journal of Bioenergetics and Biomembranes·Carrie HiserShelagh Ferguson-Miller

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