The murine female intestinal microbiota does not shift throughout the estrous cycle

PloS One
Jessica G WallaceDeborah M Sloboda

Abstract

Pregnancy is accompanied by maternal physiological adaptations including metabolic, endocrine, immune, cardiovascular, skeletomuscular and neurological modifications that facilitate fetal and placental growth and development. Emerging evidence suggests that the maternal intestinal microbiota is modified over the course of healthy pregnancy. We have recently identified a maternal intestinal microbial shift within hours of conception; a shift that continued with advancing gestation. It is possible that maternal gut bacterial profiles might be associated with the known endocrine changes that accompany the female reproductive (estrous) cycle. To determine whether the estrous cycle influenced the shifts in the maternal intestinal microbiota, time-matched fecal pellets were collected daily for 3 consecutive estrous cycles from individually housed, non-pregnant female C57BL/6J mice (n = 10) fed a control diet. Estrous stage was identified by cell type predominance in vaginal cytological samples. The corresponding fecal pellets for each estrous stage were processed for bacterial 16S rRNA sequencing of the variable 3 (V3) region. Estrous cycle stage accounted for a very small and not statistically significant proportion of the variation...Continue Reading

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Methods Mentioned

BETA
PCR

Software Mentioned

Cutadapt
dplyr
custom R scripts
AbundantOTU RDP Classifier
QIIME
Quantitative Insights into Microbial Ecology ( QIIME )
tidyr
ggplot2
phyloseq
sl1p

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