PMID: 8960105Oct 1, 1996Paper

The murine myeloperoxidase gene contains a bipartite distal enhancer, including a novel region regulated by PEBP2/CBF

Leukemia Research
A D FriedmanJ Suzow

Abstract

The myeloperoxidase (MPO) gene is expressed specifically in immature myeloid cells. DNA (27 kb) derived from the murine MPO gene was subcloned into nine segments, and each examined for its ability to enhance transcription in 32D cl3 myeloid cells. Only one segment, besides the known proximal enhancer, was active. Deletional analysis of this region delimited two functional regions. One is located 3.4 kb upstream of the proximal enhancer and was also recently identified in a different cell system. The second novel region is a 133 bp segment located 1.1 kb further upstream. This region, like the proximal enhancer, binds and is regulated by PEBP2/CBF. The PEBP2/CBF binding-site in the distal enhancer, AACCAAT, differs from the PEBP2/CBF consensus, PuACCPuCA.

Citations

Oct 1, 1996·Leukemia Research·G E AustinJ P Lu
Apr 6, 2000·Current Opinion in Oncology·H J Deeg, F R Appelbaum
Mar 3, 1998·Journal of Pediatric Hematology/oncology·D R BettsF K Niggli
Nov 11, 1999·British Journal of Haematology·P D Emanuel
Jun 26, 2002·Oncogene·Florence Bernardin, Alan D Friedman
Jan 30, 2003·Oncogene·Fernando P G SilvaMicheline Giphart-Gassler

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