PMID: 7085409Apr 1, 1982Paper

The muscarinic receptors of airway smooth muscle: their characterization in vitro

Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology
C MurlasJ M Roberts

Abstract

We have used radioligand binding and in vitro muscle contraction techniques to characterize the muscarinic cholinergic receptors of canine tracheal smooth muscle. The tritiated muscarinic antagonist, quinuclidinyl benzilate ([3H]QNB), bound no particulate preparations of tracheal smooth muscle with high affinity, saturability, pharmacologic specificity, and stereoselectivity. The equilibrium dissociation constant for the binding reaction was 33 +/- 3 pM (mean +/- SE). The concentration of binding sites was 410 +/- 34 pmol/g protein. The ability of muscarinic agents to inhibit [3H]QNB binding paralleled their relative effects on tracheal smooth muscle contraction in vitro. We found the anesthetic agents, lidocaine and tetracaine, and the nicotonic cholinergic antagonist d-tubocurarine, were competitive inhibitors of [3H]QNB binding to the tracheal smooth muscle preparations. Histamine, aminophylline, and adrenergic agonists did not act as competitive inhibitors. The guanine nucleotide, guanyl-5'-imidodiphosphate, reduced the ability of the muscarinic agonist, acetylcholine, to compete with high affinity for [3H]QNB binding site(s) in tracheal smooth muscle but had minimal effects on the binding of the muscarinic antagonist, atro...Continue Reading

Citations

May 1, 1990·Anaesthesia·C S Martin, C D Miller
Aug 1, 1989·Postgraduate Medical Journal·P J Barnes
Aug 10, 2006·Pharmaceutical Development and Technology·Michael K TaylorMichiel VanOort
Mar 1, 1986·Journal of Autonomic Pharmacology·C M YangT M Dwyer
Mar 1, 1992·American Journal of Respiratory Cell and Molecular Biology·V K MaheshJ W Bloom
May 1, 1990·Acta Anaesthesiologica Scandinavica·Y SugaiM Miyazaki
Feb 1, 1995·Hypertension·L Cao, D G Gardner
Nov 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·C J van KoppenC A van Ginneken
Jan 1, 1988·Clinical and Experimental Hypertension. Part A, Theory and Practice·C D SmithM J Katovich
Jul 19, 1996·The Journal of Biological Chemistry·A Kralli, K R Yamamoto
Apr 1, 1991·British Journal of Pharmacology·R C JongejanK F Kerrebijn
Jul 1, 1987·The American Journal of Physiology·C A JonesJ K Brown
Feb 1, 1989·The American Journal of Physiology·J M MadisonW E Finkbeiner

Related Concepts

Acetylcholine Sulfate (1: 1)
Atropen
Canis familiaris
Melting
Guanylyl Imidodiphosphate
Hydrogen-Ion Concentration
Muscle Proteins
Smooth Muscle
Quinuclidinyl Benzilate
Cholinergic Receptors

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
H I Yamamura, S H Snyder
© 2021 Meta ULC. All rights reserved