The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases.

Chemical Communications : Chem Comm
Yujing HuQi Wu

Abstract

The mutagenesis of a "second sphere" switch residue of CHMOAcineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMOAcineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).

References

Oct 27, 2006·The Journal of Organic Chemistry·Christopher M ClouthierManfred T Reetz
Apr 24, 2009·Journal of the American Chemical Society·I Ahmad MirzaAlbert M Berghuis
Jul 22, 2009·Nature Chemical Biology·Nicholas J Turner
Oct 8, 2009·Journal of the American Chemical Society·Manfred T Reetz, Sheng Wu
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sheng WuManfred T Reetz
Jun 24, 2011·The Journal of Biological Chemistry·Roberto OrruAndrea Mattevi
Jan 14, 2012·Journal of the American Chemical Society·Iakov PolyakWalter Thiel
Jun 7, 2014·Chemical Communications : Chem Comm·Dirk HoltmannFrank Hollmann
Nov 23, 2016·Angewandte Chemie·Elvira RomeroMarco W Fraaije
Dec 10, 2016·ACS Chemical Biology·Hugo L van BeekMarco W Fraaije
Mar 16, 2017·Chemical Communications : Chem Comm·Jian-Bo WangManfred T Reetz
May 16, 2017·Chembiochem : a European Journal of Chemical Biology·Kathleen BalkeUwe T Bornscheuer
Sep 7, 2017·Chemistry : a European Journal·Sandy SchmidtRobert Kourist
Nov 28, 2017·Biotechnology Advances·Kathleen BalkeUwe T Bornscheuer
Mar 2, 2018·Annual Review of Biochemistry·Cathleen Zeymer, Donald Hilvert
Mar 25, 2018·Angewandte Chemie·JiaJia DongWuyuan Zhang
Jul 26, 2018·Journal of the American Chemical Society·Guangyue LiManfred T Reetz
Jan 29, 2019·ACS Catalysis·Maximilian J L J FürstAndrea Mattevi

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Software Mentioned

CHMO
CHMO Acineto
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