The MVA pathway genes expressions and accumulation of celastrol in Tripterygium wilfordii suspension cells in response to methyl jasmonate treatment.

Journal of Asian Natural Products Research
Yu-Jia LiuWei Gao

Abstract

Celastrol is an important bioactive triterpenoid in traditional Chinese medicinal plant, Tripterygium wilfordii. Methyl Jasmonate (MJ) is a common plant hormone which can regulate the secondary metabolism in higher plants. In this study, the mevalonate (MVA) pathway genes in T. wilfordii were firstly cloned. The suspension cells of T. wilfordii were elicited by MJ, and the expressions of MVA pathway genes were all enhanced in different levels ranging from 2.13 to 22.33 times of that at 0 h. The expressions were also enhanced compared with the CK group separately. The accumulation of celastrol in the suspension cells after the treatment was quantified and co-analyzed with the genes expression levels. The production of celastrol was significantly increased to 0.742 mg g(-1) after MJ treatment in 288 h which is consistent with the genes expressions. The results provide plenty of gene information for the biosynthesis of terpenoids in T. wilfordii and a viable way to improve the accumulation of celastrol in T. wilfordii suspension cells.

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Citations

Feb 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bin ZhangChuanshu Zhu
Mar 24, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shaohua XuXianyu Li
Jul 18, 2021·Phytochemistry·Jiawei ZhouWei Gao

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Datasets Mentioned

BETA
KR297934
KM978213
AAL18924.1

Software Mentioned

ExPASy
BLAST
MEGA5

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