The MYO6 interactome reveals adaptor complexes coordinating early endosome and cytoskeletal dynamics.

EMBO Reports
Thomas O'LoughlinFolma Buss

Abstract

The intracellular functions of myosin motors requires a number of adaptor molecules, which control cargo attachment, but also fine-tune motor activity in time and space. These motor-adaptor-cargo interactions are often weak, transient or highly regulated. To overcome these problems, we use a proximity labelling-based proteomics strategy to map the interactome of the unique minus end-directed actin motor MYO6. Detailed biochemical and functional analysis identified several distinct MYO6-adaptor modules including two complexes containing RhoGEFs: the LIFT (LARG-Induced F-actin for Tethering) complex that controls endosome positioning and motility through RHO-driven actin polymerisation; and the DISP (DOCK7-Induced Septin disPlacement) complex, a novel regulator of the septin cytoskeleton. These complexes emphasise the role of MYO6 in coordinating endosome dynamics and cytoskeletal architecture. This study provides the first in vivo interactome of a myosin motor protein and highlights the power of this approach in uncovering dynamic and functionally diverse myosin motor complexes.

References

Jun 26, 1998·Science·T KozasaP C Sternweis
Oct 16, 1999·Nature·A L WellsH L Sweeney
Mar 29, 2000·The Journal of Biological Chemistry·M C SubausteK M Hahn
Oct 13, 2000·Science·V S KraynovK M Hahn
Oct 5, 2001·Nature Cell Biology·G JobertyI G Macara
Dec 14, 2002·Developmental Cell·Makoto KinoshitaTimothy J Mitchison
Jul 15, 2003·Molecular Biology of the Cell·Laura AschenbrennerTama Hasson
Mar 9, 2004·Molecular Biology of the Cell·Laura AschenbrennerTama Hasson
Sep 14, 2004·Science·Perihan NalbantKlaus M Hahn
Oct 2, 2004·Protein Science : a Publication of the Protein Society·Eunjoo Choi-RheeJohn E Cronan
Nov 16, 2004·BMC Cell Biology·Katja Schmidt, Benjamin J Nichols
Jan 20, 2005·The Journal of Cell Biology·Emily OsterweilMark S Mooseker
Apr 20, 2005·The Journal of Cell Biology·Daniela A SahlenderFolma Buss
Aug 10, 2005·The Journal of Cell Biology·Vladimir SirotkinThomas D Pollard
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Samia N NaccacheArie Horowitz
Oct 31, 2006·The American Journal of Pathology·Thomas A DunnJun Luo
Sep 21, 2007·Molecular Biology of the Cell·Susan D ArdenFolma Buss
Apr 17, 2008·Methods in Enzymology·Margarita V ChibalinaFolma Buss
Jul 11, 2008·The Journal of Biological Chemistry·Patrick J RobertsChanning J Der
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Peter J HanleyMartin Bähler
Oct 15, 2010·Protein Science : a Publication of the Protein Society·Marta H KubalaBrett M Collins
Dec 7, 2010·Nature Methods·Hyungwon ChoiAlexey I Nesvizhskii
Apr 6, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Łukasz MajewskiMaria Jolanta Rędowicz
Jun 8, 2012·Molecular Biology of the Cell·Jackie ChengDavid G Drubin
Sep 20, 2012·Development·Arnaud P GieseMireille Montcouquiol
Sep 21, 2012·The Journal of Biological Chemistry·Lisa M BondJames R Sellers
Aug 8, 2013·Nature Methods·Dattatreya MellacheruvuAlexey I Nesvizhskii
Dec 18, 2013·The International Journal of Biochemistry & Cell Biology·Juyeon Hwang, David C Pallas
Nov 26, 2014·Proteomics·James D R KnightAnne-Claude Gingras
Nov 4, 2015·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob

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Citations

Jun 18, 2019·FEBS Letters·Janeska J de JongeFolma Buss
Feb 18, 2020·Biochemical Society Transactions·Giulia ManaGuido Serini
Oct 26, 2018·Frontiers in Cellular Neuroscience·Yolanda Gimenez-MolinaLuis M Gutiérrez
May 6, 2019·ELife·Jorge Carretero-OrtegaJesús Torres-Vázquez
Aug 26, 2020·The Journal of Cell Biology·Prasad V PhatarpekarDaniel D Billadeau
Oct 20, 2020·Frontiers in Cell and Developmental Biology·Thibaud RivièreDaniela Maier-Begandt
Jan 3, 2021·Histochemistry and Cell Biology·Przemysław ZakrzewskiFolma Buss
Mar 25, 2021·Developmental Cell·Christoph J BurckhardtGaudenz Danuser
Jul 14, 2021·Trends in Cell Biology·Elias T Spiliotis, Ilona A Kesisova
Aug 28, 2021·International Journal of Molecular Sciences·Béatrice BenoitChristian Poüs
Dec 16, 2021·Journal of Cell Science·Anita BailletPatrick W Oakes

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Datasets Mentioned

BETA
PXD008686

Methods Mentioned

BETA
immunoprecipitation
confocal microscopy
GTPases
M2H
PCR
transfections
transfection
Protein Assay
acetylation

Software Mentioned

Andromeda
R
BioID
Cytoscape
MaxQuant
org
ProHits
ImageJ
CRAPome
GraphPad Prism

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