PMID: 3755847Jan 1, 1986Paper

The myosin filament. X. Observation of nine subfilaments in transverse sections

Tissue & Cell
F A PepeJ Weisel

Abstract

The molecular packing of the subfilaments in muscle thick filaments has been investigated by electron microscopy. Thin (80-100 nm) transverse sections of vertebrate skeletal muscle were cut, and 129 electron microscope images of thick filaments from 15 different areas including seven to ten images in each area were analyzed by computer image processing. The transverse sections were limited to the portion of the filaments between the bare zone and the C-protein bearing region. Of the 129 images, six were discarded because they were structurally disrupted, 17 did not show evidence for the presence of subfilaments from the autocorrelation function, and four did not show evidence for three-fold rotational symmetry from the power spectrum. The remaining 102 filaments all showed evidence for three-fold rotational symmetry, consistent with other available evidence (Pepe, 1982). From the analysis of these images by rotational filtering, we have found that the vertebrate skeletal myosin filament is made up of nine subfilaments and that the image appears to have trigonal symmetry. Of these subfilaments, six are arranged with a center-to-center spacing of about 4 nm and the other three on the surface of the filament are distorted from thi...Continue Reading

References

Dec 25, 1975·Journal of Molecular Biology·F A Pepe, B Drucker
Feb 1, 1972·The Journal of Cell Biology·F A Pepe, B Drucker
Aug 28, 1971·Journal of Molecular Biology·R A Crowther, L A Amos
Jun 1, 1983·The Journal of Cell Biology·R W Kensler, M Stewart
Jul 24, 1980·Nature·M C Maw, A J Rowe
Jan 15, 1981·Journal of Molecular Biology·F A PepeM Stewart

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Citations

Aug 1, 1989·The Journal of Cell Biology·P Vibert, L Castellani
Apr 17, 2010·Journal of Biomedicine & Biotechnology·Maegen A Ackermann, Aikaterini Kontrogianni-Konstantopoulos
Oct 27, 2011·Journal of Biomedicine & Biotechnology·Maegen A Ackermann, Aikaterini Kontrogianni-Konstantopoulos
Feb 24, 1989·Biochimica Et Biophysica Acta·P K ChowrashiF A Pepe
Jul 17, 2015·Journal of Cardiovascular Magnetic Resonance : Official Journal of the Society for Cardiovascular Magnetic Resonance·Russell DibbChunlei Liu
Sep 2, 1998·Journal of Structural Biology·J SquireP Luther
Jan 29, 2000·Microscopy Research and Technique·M RoyuelaR Paniagua
Feb 1, 1989·Journal of Muscle Research and Cell Motility·R Ward, P M Bennett
Apr 1, 1992·Journal of Muscle Research and Cell Motility·L Castellani, P Vibert
Oct 1, 1990·Journal of Muscle Research and Cell Motility·R Ward, J M Murray
Aug 31, 1989·Biochimica Et Biophysica Acta·P K Chowrashi, F A Pepe

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