PMID: 9436992Mar 14, 1998Paper

The myosin I SH3 domain and TEDS rule phosphorylation site are required for in vivo function

Molecular Biology of the Cell
K D Novak, Margaret A Titus

Abstract

The class I myosins play important roles in controlling many different types of actin-based cell movements. Dictyostelium cells either lacking or overexpressing amoeboid myosin Is have significant defects in cortical activities such as pseudopod extension, cell migration, and macropinocytosis. The existence of Dictyostelium null mutants with strong phenotypic defects permits complementation analysis as a means of exploring important functional features of the myosin I heavy chain. Mutant Dictyostelium cells lacking two myosin Is exhibit profound defects in growth, endocytosis, and rearrangement of F-actin. Expression of the full-length myoB heavy chain in these cells fully rescues the double mutant defects. However, mutant forms of the myoB heavy chain in which a serine at the consensus phosphorylation site has been altered to an alanine or in which the C-terminal SH3 domain has been removed fail to complement the null phenotype. The wild-type and mutant forms of the myoB heavy chain appeared to be properly localized when they were expressed in the myosin I null mutants. These results suggest that the amoeboid myosin I consensus phosphorylation site and SH3 domains do not play a role in the localization of myosin I, but are abs...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A Kuspa, W F Loomis
Oct 30, 1992·Cell·T Pawson, G D Gish
Jan 1, 1991·Cell Motility and the Cytoskeleton·D WesselsD R Soll
Jan 1, 1991·Annual Review of Physiology·T D PollardH G Zot
Aug 14, 1986·Biochemical and Biophysical Research Communications·G Klein, M Satre
Aug 17, 1989·Nature·R J Adams, T D Pollard
Oct 1, 1989·The Journal of Cell Biology·H MiyataE D Korn
Mar 25, 1988·Nucleic Acids Research·P K HowardR A Firtel
Aug 31, 1987·FEBS Letters·V M WaseniusM Saraste
Feb 16, 1995·Nature·T Pawson
Dec 1, 1995·The Journal of Cell Biology·K D NovakM A Titus
Mar 1, 1996·The Journal of Cell Biology·E M Ostap, T D Pollard
Apr 1, 1996·The Journal of Cell Biology·E M Ostap, T D Pollard
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M S Mooseker, R E Cheney
Jul 19, 1996·The Journal of Biological Chemistry·H Brzeska, E D Korn
Dec 13, 1996·The Journal of Biological Chemistry·C WuE Leberer
Feb 10, 1997·The Journal of Cell Biology·K D Novak, M A Titus
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·P XuH G Zot

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Citations

Jul 14, 1999·Cell Biochemistry and Biophysics·T SoldatiE C Schwarz
Mar 21, 2001·Biochimica Et Biophysica Acta·M A de la Roche, G P Côté
Feb 19, 2000·Current Opinion in Cell Biology·X WuJ A Hammer
Mar 1, 1997·Trends in Cell Biology·M A Titus
Mar 1, 2012·The Journal of Biological Chemistry·Hanna BrzeskaEdward D Korn
Oct 29, 2004·Molecular Biology of the Cell·Marc de la RocheGraham P Côté
Dec 3, 2008·Eukaryotic Cell·Stacey D GilkCon J M Beckers
Nov 29, 2002·Eukaryotic Cell·U OberholzerM Whiteway
Oct 8, 2004·Eukaryotic Cell·Ursula OberholzerMalcolm Whiteway
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z Y WangJ A Hammer
Jun 10, 2000·The Journal of Eukaryotic Microbiology·M A Titus
Dec 4, 2012·Trends in Cell Biology·Michael J Greenberg, E Michael Ostap
Aug 19, 2006·Cell Motility and the Cytoskeleton·Samia N Naccache, Tama Hasson
Apr 8, 2009·Biophysical Journal·Till BretschneiderGünther Gerisch
Aug 18, 2001·Current Biology : CB·N Tang, E M Ostap
Mar 21, 2000·Biochimica Et Biophysica Acta·B BarylkoJ P Albanesi
Jul 29, 1999·Biophysical Journal·J DaiM A Titus
Nov 1, 2000·Cell Motility and the Cytoskeleton·N Osherov, G S May
Jan 24, 2016·Cytoskeleton·Hanna BrzeskaMargaret A Titus
Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M ToyaM Yamamoto
Nov 13, 2007·Journal of Muscle Research and Cell Motility·Nanyun TangE Michael Ostap
Mar 2, 2019·Journal of Leukocyte Biology·Daniel Alberto Girón-PérezLeopoldo Santos-Argumedo
Dec 20, 2007·The Journal of Biological Chemistry·Georgios TsiavaliarisDietmar J Manstein
Aug 5, 2017·Acta Crystallographica. Section D, Structural Biology·Gunjan GautamSamudrala Gourinath
Nov 21, 2000·The Journal of Biological Chemistry·N R GliksmanM A Titus
Jan 31, 2006·Journal of Cell Science·Ulrike DürrwangDietmar J Manstein
Mar 21, 2001·Biochimica Et Biophysica Acta·S MaR L Chisholm

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