The N- and C-terminal domains of parathyroid hormone-related protein affect differently the osteogenic and adipogenic potential of human mesenchymal stem cells.

Experimental & Molecular Medicine
Antonio Casado-DiazJosé Manuel Quesada

Abstract

Parathyroid hormone-related protein (PTHrP) is synthesized by diverse tissues, and its processing produces several fragments, each with apparently distinct autocrine and paracrine bioactivities. In bone, PTHrP appears to modulate bone formation in part through promoting osteoblast differentiation. The putative effect of PTH-like and PTH-unrelated fragments of PTHrP on human mesenchymal stem cell (MSCs) is not well known. Human MSCs were treated with PTHrP (1-36) or PTHrP (107-139) or both (each at 10 nM) in osteogenic or adipogenic medium, from the start or after 6 days of exposure to the corresponding medium, and the expression of several osteoblastogenic and adipogenic markers was analyzed. PTHrP (1-36) inhibited adipogenesis in MSCs and favoured the expression of osteogenic early markers. The opposite was observed with treatment of MSCs with PTHrP (107-139). Moreover, inhibition of the adipogenic differentiation by PTHrP (1-36) prevailed in the presence of PTHrP (107-139). The PTH/PTHrP type 1 receptor (PTH1R) gene expression was maximum in the earlier and later stages of osteogenesis and adipogenesis, respectively. While PTHrP (107-139) did not modify the PTH1R overexpression during adipogenesis, PTHrP (1-36) did inhibit it...Continue Reading

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Citations

Mar 29, 2014·Journal of Mid-life Health·Sudhaa SharmaAnnil Mahajan
May 13, 2014·Cell and Tissue Research·Oliver FelthausChristian Morsczeck
May 5, 2012·Biochemical and Biophysical Research Communications·Jong-Min Lee, Gun-Il Im
Aug 26, 2014·Biochimica Et Biophysica Acta·Kamil BorkowskiKarsten Kristiansen
Apr 8, 2016·Physiology·Jawed A Siddiqui, Nicola C Partridge

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