PMID: 9183011May 1, 1997Paper

The N-domain of the signal recognition particle 54-kDa subunit promotes efficient signal sequence binding

European Journal of Biochemistry
J A Newitt, H D Bernstein

Abstract

The signal recognition particle 54-kDa subunit (SRP54) binds to the signal sequences of nascent presecretory and transmembrane proteins. Previous studies have shown that signal sequences bind to the C-terminal methionine-rich domain of the protein (M-domain), but have raised the possibility that either the N-terminal domain (N-domain) or the central guanosine triphosphatase module (GTPase-domain) also contribute to signal-sequence-binding activity. We have generated a series of N-domain and GTPase-domain mutants to investigate this issue further. Mutations in a conserved N-domain motif (ALLEADV) produced significant defects in signal sequence binding that correlate with the severity of the mutation. The magnitude of the defect was independent of the preprotein substrate, which suggested that the mutations do not alter the specificity of signal sequence recognition. The N-domain mutants also showed defects in promoting the translocation of presecretory proteins across the membrane of microsomal vesicles, but these defects appeared to be a direct consequence of the reduction in signal-sequence-binding activity and not separate effects of the mutations. By contrast, mutations in the guanosine triphosphatase consensus sequence had ...Continue Reading

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Citations

Jul 4, 2002·Journal of Molecular Biology·Ursula D RamirezDouglas M Freymann
May 16, 2002·Biochimica Et Biophysica Acta·Irina V Shepotinovskaya, Douglas M Freymann
Feb 13, 2003·Current Opinion in Structural Biology·A Elisabeth Sauer-Eriksson, Tobias Hainzl
Dec 14, 2012·Nature·Harris D Bernstein
Sep 12, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tobias HainzlA Elisabeth Sauer-Eriksson
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gert BangeIrmgard Sinning
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ken R RosendalIrmgard Sinning
Jan 17, 2004·Science·Pamela J FociaDouglas M Freymann
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Dec 15, 2007·Annual Review of Biochemistry·Arnold J M Driessen, Nico Nouwen
Jul 31, 2010·Archaea : an International Microbiological Journal·Christian Zwieb, Shakhawat Bhuiyan
Jul 2, 2004·Molecular Microbiology·Klemens WildIrmgard Sinning
Dec 14, 2005·Journal of Structural Biology·Talal GarianiA Elisabeth Sauer-Eriksson
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Dec 3, 2016·Current Microbiology·Sayandeep GuptaAbhrajyoti Ghosh
Oct 21, 1998·Current Biology : CB·H D Bernstein

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