The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (alpha 1-3)galactose units

Molecular and Biochemical Parasitology
A S CoutoR M de Lederkremer

Abstract

The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [14C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding to the lectin was lost on treatment of Tc-85 with neuraminidase. The N-linked asialo-oligosaccharide was released by endo-beta-N-acetylglucosaminidase F digestion of asialo-Tc-85 and was further analyzed using specific exoglycosidases. [14C]fucose was detected after alpha-L-fucosidase treatment or mild acid hydrolysis. The afucosyl oligosaccharide was 3H-labeled by the galactose oxidase-NaB3H4 method. [3H]Galactose was released by alpha-galactosidase, and only then was beta-galactosidase effective in removing another galactose. The gal(alpha 1-3)gal unit was demonstrated by periodate oxidation studies on the [3H]galactose-labeled asialo-glycoprotein. The presence of gal(alpha 1-3)gal in Tc-85 could be related to the recent finding of elevated antibody levels against this epitope in patients with Chagas' disease.

References

Sep 1, 1987·Molecular and Biochemical Parasitology·C F IbañezA C Frasch
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G HarthM So
Jul 1, 1988·Molecular and Biochemical Parasitology·C F IbañezA C Frasch

❮ Previous
Next ❯

Citations

May 30, 2001·Molecular and Biochemical Parasitology·A Acosta-SerranoS Schenkman
Jan 6, 2006·Parasitology·D M McKay
Nov 26, 2011·Journal of Proteome Research·Ernesto S NakayasuIgor C Almeida
Apr 15, 1993·European Journal of Biochemistry·M A Ferrero-GarcíaA J Parodi
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Malcolm J McConvilleRohan D Teasdale
Apr 9, 2016·PLoS Pathogens·Andrés B LantosOscar Campetella
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Maria Julia Manso Alves, Renato Arruda Mortara
Apr 26, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L C MilettiM J M Alves
Jan 18, 2016·Trends in Parasitology·Miguel P Soares, Bahtiyar Yilmaz
Jun 10, 2000·Memórias do Instituto Oswaldo Cruz·W Colli, M J Alves
Dec 20, 2017·Frontiers in Immunology·Alejandro Cabezas-Cruz, José de la Fuente
Dec 1, 1991·Glycoconjugate Journal·S Zamze
Oct 1, 2020·Molecular & Cellular Proteomics : MCP·Christopher M WestIain B H Wilson
Mar 1, 1993·Infection and Immunity·R P SchenkmanS Schenkman
Dec 5, 2017·ACS Central Science·Alejandro Cabezas-Cruz, José de la Fuente
Jun 8, 2011·Carbohydrate Research·M Eugenia Giorgi, Rosa M de Lederkremer

❮ Previous
Next ❯

Related Concepts