The N-terminal and transmembrane domains of Commissureless are necessary for its function and trafficking within neurons
Commissureless (Comm) is a novel transmembrane molecule necessary both for commissural axons to cross the midline of the Drosophila central nervous system and normal synaptogenesis. Comm is able to reduce cell surface levels of Roundabout (Robo), a receptor for the midline repellent Slit, on commissural axons and unknown inhibitors of synaptogenesis expressed on muscle cells. Comm is expressed dynamically and is found at the cell surface and within intracellular vesicles. Comm can bind Robo and when the proteins are co-expressed Robo is found co-localised with Comm intracellularly. Here we show that the ability of Comm to localise intracellularly and hence regulate Robo surface levels requires sequences in both the N-terminal and transmembrane domains. We also show that Comm can dimerise via its N-terminal domain. Furthermore, absence of the Comm N-terminal and transmembrane regions results in the protein being restricted to the neuron soma.
The midline of the Drosophila central nervous system: a model for the genetic analysis of cell fate, cell migration, and growth cone guidance
The isolation and sequence of the gene encoding T8: a molecule defining functional classes of T lymphocytes
Imaging neuronal subsets and other cell types in whole-mount Drosophila embryos and larvae using antibody probes
Trafficking of cell surface beta-amyloid precursor protein: retrograde and transcytotic transport in cultured neurons
Mutations affecting growth cone guidance in Drosophila: genes necessary for guidance toward or away from the midline
commissureless controls growth cone guidance across the CNS midline in Drosophila and encodes a novel membrane protein
Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors
Dosage-sensitive and complementary functions of roundabout and commissureless control axon crossing of the CNS midline
Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance
Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
Maturation of the axonal plasma membrane requires upregulation of sphingomyelin synthesis and formation of protein-lipid complexes
Apical membrane targeting of Nedd4 is mediated by an association of its C2 domain with annexin XIIIb
Squeezing axons out of the gray matter: a role for slit and semaphorin proteins from midline and ventral spinal cord
Hierarchical organization of guidance receptors: silencing of netrin attraction by slit through a Robo/DCC receptor complex
Drosophila Nedd4, a ubiquitin ligase, is recruited by Commissureless to control cell surface levels of the roundabout receptor
Regulation of Commissureless by the ubiquitin ligase DNedd4 is required for neuromuscular synaptogenesis in Drosophila melanogaster
The cell surface receptor Tartan is a potential in vivo substrate for the receptor tyrosine phosphatase Ptp52F.
In the absence of frazzled over-expression of Abelson tyrosine kinase disrupts commissure formation and causes axons to leave the embryonic CNS.
Brain developing: Influences & Outcomes
This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.