The N-terminal ATPase AT-hook-containing region of the Arabidopsis chromatin-remodeling protein SPLAYED is sufficient for biological activity

The Plant Journal : for Cell and Molecular Biology
Yanhui SuDoris Wagner

Abstract

The SNF2-like chromatin-remodeling ATPase SPLAYED (SYD) was identified as a co-activator of floral homeotic gene expression in Arabidopsis. SYD is also required for meristem maintenance and regulates flowering under a non-inductive photoperiod. SNF2 ATPases are structurally and functionally conserved from yeast to humans. In addition to the conserved protein features, SYD has a large unique C-terminal domain. We show here that SYD is present as two forms in the nucleus, full-length and truncated, with the latter apparently lacking the C-terminal domain. The ratio of the two forms of endogenous SYD differs in juvenile and in adult tissues. Furthermore, an SYD variant lacking the C-terminal domain (SYDDeltaC) rescues the syd null mutant, indicating that the N-terminal ATPase AT-hook-containing region of SYD is sufficient for biological activity. Plants expressing SYDDeltaC show molecular and morphological phenotypes opposite to those of the null mutant, suggesting that the construct results in increased activity. This increased activity is at least in part due to elevated SYD protein levels in these lines. We propose that the C-terminal domain may control SYD accumulation and/or specific activity in the context of the full-length...Continue Reading

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Citations

Feb 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Miin-Feng WuDoris Wagner
Sep 13, 2008·Trends in Plant Science·Lukasz KnizewskiAndrzej Jerzmanowski
Jun 15, 2007·Trends in Genetics : TIG·Chang Seob Kwon, Doris Wagner
Feb 13, 2007·Biochimica Et Biophysica Acta·Andrzej Jerzmanowski
May 16, 2015·The Plant Journal : for Cell and Molecular Biology·Soon-Ki HanDoris Wagner
May 19, 2018·The Plant Journal : for Cell and Molecular Biology·Xiaowei LinSujuan Cui

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