PMID: 9005448Dec 24, 1996Paper

The N-terminal domain of a K+ channel beta subunit increases the rate of C-type inactivation from the cytoplasmic side of the channel

Proceedings of the National Academy of Sciences of the United States of America
M J MoralesR L Rasmusson

Abstract

Voltage-gated K+ channels are complexes of membrane-bound, ion-conducting alpha and cytoplasmic ancillary (beta) subunits. The primary physiologic effect of coexpression of alpha and beta subunits is to increase the intrinsic rate of inactivation of the alpha subunit. For one beta subunit, Kv beta 1.1, inactivation is enhanced through an N-type mechanism. A second beta subunit, Kv beta 1.2, has been shown to increase inactivation, but through a distinct mechanism. Here we show that the degree of enhancement of Kv beta 1.2 inactivation is dependent on the amino acid composition in the pore mouth of the alpha subunit and the concentration of extracellular K+. Experimental conditions that promote C-type inactivation also enhance the stimulation of inactivation by Kv beta 1.2, showing that this beta subunit directly stimulates C-type inactivation. Chimeric constructs containing just the nonconserved N-terminal region of Kv beta 1.2 fused with an alpha subunit behave in a similar fashion to coexpressed Kv beta 1.2 and alpha subunit. This shows that it is the N-terminal domain of Kv beta 1.2 that mediates the increase in C-type inactivation from the cytoplasmic side of the pore. We propose a model whereby the N terminus of Kv beta 1....Continue Reading

References

Dec 1, 1995·The Journal of Biological Chemistry·S K EnglandM M Tamkun
Aug 1, 1994·Analytical Biochemistry·I D Pokrovskaya, V V Gurevich
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·V E ScottJ O Dolly
Mar 22, 1996·The Journal of Biological Chemistry·K NakahiraJ S Trimmer
Apr 1, 1996·Biophysical Journal·J T Groves, H M McConnell

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Citations

Nov 30, 2000·Trends in Cardiovascular Medicine·J R MartensM M Tamkun
Feb 28, 2004·Trends in Cardiovascular Medicine·J Xu, M Li
Apr 14, 2004·The Journal of General Physiology·Harley T KurataDavid Fedida
Aug 8, 2008·Journal of Neurophysiology·Ricardo H PinedaAngeles B Ribera
Jun 17, 2010·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Won Sun ParkEun A Ko
Mar 1, 2011·The Biochemical Journal·Ahmed Al-SabiJ Oliver Dolly
Nov 11, 2003·The Journal of Physiology·Glenna C L Bett, Randall L Rasmusson
Apr 30, 1998·Circulation Research·R L RasmussonH C Strauss
Jun 6, 2015·Circulation Research·Hugues AbrielJosé Jalife
Jun 2, 2006·American Journal of Physiology. Cell Physiology·Hong-Ling LiMichael J Morales
Dec 22, 2007·The Journal of Physiology·Glenna C L Bett, Randall L Rasmusson
Jan 24, 2002·The Journal of Pharmacology and Experimental Therapeutics·David GodreauStéphane N Hatem
Dec 18, 1998·The Journal of Biological Chemistry·T LeicherO Pongs
Jun 1, 2002·International Immunology·Chiguang FengPaul E Love
Jun 25, 2020·The Journal of General Physiology·Eric Accili
Apr 21, 2020·The Journal of General Physiology·Shawn M Lamothe, Harley T Kurata
Oct 3, 1999·Physiological Reviews·F Lehmann-Horn, K Jurkat-Rott
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·E A CoppockM M Tamkun
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Xiaoyan LiRandall L Rasmusson
Dec 31, 2010·Biophysical Journal·Glenna C L BettRandall L Rasmusson

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