PMID: 9636150Jun 24, 1998Paper

The N-terminal domain of PsaF: precise recognition site for binding and fast electron transfer from cytochrome c6 and plastocyanin to photosystem I of Chlamydomonas reinhardtii

Proceedings of the National Academy of Sciences of the United States of America
M HipplerJean-David Rochaix

Abstract

The PsaF-deficient mutant 3bF of Chlamydomonas reinhardtii was used to modify PsaF by nuclear transformation and site-directed mutagenesis. Four lysine residues in the N-terminal domain of PsaF, which have been postulated to form the positively charged face of a putative amphipathic alpha-helical structure were altered to K12P, K16Q, K23Q, and K30Q. The interactions between plastocyanin (pc) or cytochrome c6 (cyt c6) and photosystem I (PSI) isolated from wild type and the different mutants were analyzed using crosslinking techniques and flash absorption spectroscopy. The K23Q change drastically affected crosslinking of pc to PSI and electron transfer from pc and cyt c6 to PSI. The corresponding second order rate constants for binding of pc and cyt c6 were reduced by a factor of 13 and 7, respectively. Smaller effects were observed for mutations K16Q and K30Q, whereas in K12P the binding was not changed relative to wild type. None of the mutations affected the half-life of the microsecond electron transfer performed within the intermolecular complex between the donors and PSI. The fact that these single amino acid changes within the N-terminal domain of PsaF have different effects on the electron transfer rate constants and diss...Continue Reading

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Citations

Jun 25, 2005·Photosynthesis Research·Ingo Grotjohann, Petra Fromme
Jul 27, 2007·Cell Biochemistry and Biophysics·Kvetoslava Burda
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Parag R Chitnis
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May 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Giovanni FinazziMichael Hippler
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Jun 24, 2011·Bioresource Technology·Martin WinklerThomas Happe
Feb 15, 2008·Plant Physiology and Biochemistry : PPB·Alexey Amunts, Nathan Nelson
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