The N-terminal domain of the tomato immune protein Prf contains multiple homotypic and Pto kinase interaction sites.

The Journal of Biological Chemistry
Isabel M L SaurJohn P Rathjen

Abstract

Resistance to Pseudomonas syringae bacteria in tomato (Solanum lycopersicum) is conferred by the Prf recognition complex, composed of the nucleotide-binding leucine-rich repeats protein Prf and the protein kinase Pto. The complex is activated by recognition of the P. syringae effectors AvrPto and AvrPtoB. The N-terminal domain is responsible for Prf homodimerization, which brings two Pto kinases into close proximity and holds them in inactive conformation in the absence of either effector. Negative regulation is lost by effector binding to the catalytic cleft of Pto, leading to disruption of its P+1 loop within the activation segment. This change is translated through Prf to a second Pto molecule in the complex. Here we describe a schematic model of the unique Prf N-terminal domain dimer and its interaction with the effector binding determinant Pto. Using heterologous expression in Nicotiana benthamiana, we define multiple sites of N domain homotypic interaction and infer that it forms a parallel dimer folded centrally to enable contact between the N and C termini. Furthermore, we found independent binding sites for Pto at either end of the N-terminal domain. Using the constitutively active mutant ptoL205D, we identify a potent...Continue Reading

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Citations

Nov 26, 2015·Current Opinion in Microbiology·Madiha KhanDarrell Desveaux
Mar 6, 2016·Proceedings of the National Academy of Sciences of the United States of America·Isabel M L SaurJohn P Rathjen
May 12, 2016·Seminars in Cell & Developmental Biology·Karl J SchreiberJennifer D Lewis
May 22, 2016·Seminars in Cell & Developmental Biology·Octavina C A SukartaAska Goverse
Oct 9, 2018·Molecular Plant-microbe Interactions : MPMI·Takaki MaekawaPaul Schulze-Lefert
May 12, 2020·The Plant Journal : for Cell and Molecular Biology·Carolina Mazo-MolinaGregory B Martin
Jun 20, 2020·Current Opinion in Plant Biology·Joanna M FeehanJonathan Dg Jones

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