The N-terminal fingers of chicken GATA-2 and GATA-3 are independent sequence-specific DNA binding domains

The EMBO Journal
Paolo V PedoneG Felsenfeld

Abstract

The GATA family of vertebrate DNA binding regulatory proteins are expressed in diverse tissues and at different times of development. However, the DNA binding regions of these proteins possess considerable homology and recognize a rather similar range of DNA sequence motifs. DNA binding is mediated through two domains, each containing a zinc finger. Previous results have led to the conclusion that although in some cases the N-terminal finger can contribute to specificity and strength of binding, it does not bind independently, whereas the C-terminal finger is both necessary and sufficient for binding. Here we show that although this is true for the N-terminal finger of GATA-1, those of GATA-2 and GATA-3 are capable of strong independent binding with a preference for the motif GATC. Binding requires the presence of two basic regions located on either side of the N-terminal finger. The absence of one of these near the GATA-1 N-terminal finger probably accounts for its inability to bind. The combination of a single finger and two basic regions is a new variant of a motif that has been previously found in the binding domains of other finger proteins. Our results suggest that the DNA binding properties of the N-terminal finger may h...Continue Reading

References

Jan 1, 1992·Developmental Immunology·M OosterwegelH Clevers
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y H Fu, G A Marzluf
Nov 10, 1995·Journal of Molecular Biology·Y H ShimT Blumenthal
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J G OmichinskiG Felsenfeld
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·P V PedoneJ G Omichinski

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Citations

May 4, 2013·Journal of Genetics·Josiane Baccarin TraldiOrlando Moreira-Filho
Sep 23, 2003·Biochimica Et Biophysica Acta·Ferenc EvanicsRathindra N Bose
Jul 31, 2010·The Journal of Biological Chemistry·Emery H BresnickSunduz Keles
Apr 16, 2004·Plant Physiology·José C ReyesFrancisco J Florencio
Sep 19, 2006·Molecular and Cellular Biology·Jeffrey A GrassEmery H Bresnick
Apr 3, 2001·Molecular and Cellular Biology·S Ranganath, K M Murphy
Sep 7, 2012·Genome Biology·Troy W WhitfieldZhiping Weng
May 17, 2011·Journal of Molecular Endocrinology·Ann LoLisa M Halvorson
Mar 21, 2003·Biophysical Chemistry·Gary Felsenfeld
Sep 28, 2004·Journal of Cellular Biochemistry·Mako NakamuraCharles G Sagerström
Mar 11, 2008·Biochemical and Biophysical Research Communications·Tyson R VonderfechtMichael J Pikaart
Nov 27, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Hao YuDi Liu
Aug 4, 2015·Protein Science : a Publication of the Protein Society·Lorna Wilkinson-WhiteJacqueline M Matthews
Jul 14, 2009·Developmental Biology·Antonio del Castillo-OlivaresHarold E Smith
Mar 6, 2004·Journal of Leukocyte Biology·Fumihiko HayakawaHidehiko Saito
Oct 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Koichi R KatsumuraEmery H Bresnick
May 12, 2005·Journal of Cellular Physiology·Emery H BresnickKirby D Johnson
Mar 15, 2018·Nature Communications·Motoki TakakuPaul A Wade
Jun 21, 2019·Journal of Mammary Gland Biology and Neoplasia·Lisette M CornelissenJos Jonkers
Oct 2, 2019·IUBMB Life·Oriana Romano, Annarita Miccio
Jul 11, 2001·The Journal of Biological Chemistry·A NewtonM Crossley
May 16, 2000·The Journal of Biological Chemistry·D SeshasayeeD M Wojchowski
Mar 15, 2002·The Journal of Biological Chemistry·Cecile Lavenu-BombledIsabelle Max-Audit
Nov 7, 1998·The Journal of Biological Chemistry·J P MackayM Crossley
Dec 5, 1998·The Journal of Biological Chemistry·A H FoxM Crossley
Aug 28, 2003·The Journal of Biological Chemistry·Rodolfo Ghirlando, Cecelia D Trainor
Feb 27, 2004·The Journal of Biological Chemistry·M Andrew NesbitRajesh V Thakker
Mar 22, 2007·The Journal of Biological Chemistry·Ritsuko ShimizuMasayuki Yamamoto
Oct 16, 2012·Molecular Biology Reports·Wan RuiHouyan Song
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naofumi TakemotoShoichiro Miyatake
Feb 2, 2019·The Journal of Clinical Investigation·Jane E Churpek, Emery H Bresnick
Jun 23, 2000·The Journal of Biological Chemistry·C D TrainorM A Simpson

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