The N-terminal hydrophobic region of the mature phosphate translocator is sufficient for targeting to the chloroplast inner envelope membrane

The Plant Cell
J S Knight, J C Gray

Abstract

To locate the sequence required for directing the phosphate translocator to the chloroplast inner envelope membrane, a series of chimeric proteins constituting parts of the phosphate translocator and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, which is normally located in the stroma, has been produced. Reciprocal exchanges of the presequences and mature sequences of the phosphate translocator and the small subunit indicated that the phosphate translocator presequence contains stromal targeting information and that the mature protein is responsible for inner envelope membrane targeting. Chimeric proteins containing the N-terminal 46 amino acid residues of the phosphate translocator were directed to the inner envelope membrane. Subdivision of this region into its composite hydrophilic and hydrophobic regions showed that the hydrophobic region alone, which consists of amino acid residues 24 to 45, was able to direct the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase to the inner envelope membrane.

References

Jan 15, 1978·Archives of Biochemistry and Biophysics·S Murakami, H Strotmann
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1992·Trends in Biochemical Sciences·B S GlickG Schatz
Nov 13, 1991·Biochimica Et Biophysica Acta·A Douwe de Boer, P J Weisbeek
Apr 22, 1991·FEBS Letters·Y GavelG von Heijne
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M SalomonJ Soll
Jul 1, 1988·European Journal of Biochemistry·G von Heijne, Y Gavel
Jan 1, 1973·Journal of Supramolecular Structure·T L Steck, J Yu

❮ Previous
Next ❯

Citations

Oct 1, 1996·Plant Molecular Biology·M Bar-PeledN V Raikhel
Mar 3, 2010·The Journal of Biological Chemistry·Antonio A B VianaDanny J Schnell
Apr 24, 1999·The Plant Cell·K Keegstra, K Cline
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Ulf-Ingo Flugge
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Cline, R Henry
Nov 15, 2000·Biological Chemistry·U C Vothknecht, J Soll
Mar 3, 2010·Annual Review of Plant Biology·Hsou-min Li, Chi-Chou Chiu
Oct 25, 2006·The Journal of Cell Biology·Ming Li, Danny J Schnell
Aug 7, 2010·Journal of Molecular Biology·Tihana BiondaEnrico Schleiff
Nov 8, 2008·Current Opinion in Plant Biology·Kenneth Cline, Carole Dabney-Smith
Feb 12, 2009·The Plant Journal : for Cell and Molecular Biology·Jonathan R MartinKenneth Cline
Dec 17, 2008·The New Phytologist·Paul Jarvis
Jul 29, 2008·The Plant Journal : for Cell and Molecular Biology·Chi-Chou Chiu, Hsou-Min Li
Feb 18, 2009·The FEBS Journal·Kimihiro Terasawa, Naoki Sato
Jun 23, 2011·Chembiochem : a European Journal of Chemical Biology·Bettina Bölter, Jürgen Soll
Dec 10, 2009·Molecular Plant·Senthil Kumar A NatesanJohn C Gray
Dec 29, 2004·Current Biology : CB·Paul Jarvis, Colin Robinson
Aug 5, 2010·Biochimica Et Biophysica Acta·Serena SchwenkertBettina Bölter
Sep 18, 2016·Plant Physiology and Biochemistry : PPB·Fareen SamiShamsul Hayat
May 20, 2005·Molecular & Cellular Proteomics : MCP·Anne von ZychlinskiWilhelm Gruissem
Oct 11, 2017·Journal of Experimental Botany·Rajneesh Singhal, Donna E Fernandez
Sep 28, 2016·Ultrasonography·Johannes RübenthalerDirk-André Clevert
Jun 10, 2009·Nature Reviews. Genetics·Jeferson Gross, Debashish Bhattacharya
Oct 7, 2018·Photosynthesis Research·Philip M Day, Steven M Theg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.