The N-terminal presequence from F1 -ATPase β-subunit of Nicotiana plumbaginifolia efficiently targets green fluorescent fusion protein to the mitochondria in diverse commercial crops

Functional Plant Biology : FPB
Annathurai GnanasambandamStevens M Brumbley

Abstract

Approximately 10-15% of plant nuclear genes appear to encode mitochondrial proteins that are directed to mitochondria by specific targeting signals. Reports on the heterologous function of these targeting signals are generally limited to one or a few species, with an emphasis on model plants such as tobacco and Arabidopsis. Given their sequence diversity and their insufficient testing in commercially important crops (including monocotyledonous crops), the extent to which these signals can be relied on for biotechnological purposes across species remains to be established. This study provides the experimental verification of a mitochondrial signal that is functional across diverse crop species, including five monocots (sugarcane, wheat, corn, sorghum and onion) and seven dicots (cucumber, cauliflower, tomato, capsicum, pumpkin, coriander and sunflower). In all 12 crops, transient assays following microprojectile bombardment showed that the N-terminal mitochondrial presequence from F1-ATPase β-subunit (ATPase-β) of Nicotiana plumbaginifolia Viv. targeted green fluorescent fusion protein to the mitochondria. The transient assay results in sugarcane were confirmed in stably transformed root cells. The ATPase-β signal should be a us...Continue Reading

References

Aug 1, 1996·The Plant Journal : for Cell and Molecular Biology·M HayashiM Nishimura
Mar 1, 1997·The Plant Journal : for Cell and Molecular Biology·R H KöhlerM R Hanson
Jul 16, 1999·The Plant Journal : for Cell and Molecular Biology·Y NiwaH Kobayashi
Nov 19, 2003·Biochemical and Biophysical Research Communications·Françoise Ambard-BrettevilleCatherine Colas des Francs-Small
Jun 1, 2005·The Plant Cell·Itsara PracharoenwattanaSteven M Smith
Sep 7, 2005·Trends in Cell Biology·Sally A Mackenzie
Mar 21, 2006·Biochimica Et Biophysica Acta·David C Logan
Dec 16, 2006·Plant Biotechnology Journal·Richard B McQualterStevens M Brumbley
Jan 9, 2007·Plant Biotechnology Journal·Lars A PetrasovitsStevens M Brumbley
Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann

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