The N-terminal pro region mediates retention of unprocessed type-I PME in the Golgi apparatus

The Plant Journal : for Cell and Molecular Biology
Sebastian WolfSteffen Greiner

Abstract

The pectin matrix of the cell wall, a complex and dynamic network, impacts on cell growth, cell shape and signaling processes. A hallmark of pectin structure is the methylesterification status of its major component, homogalacturonan (HGA), which affects the biophysical properties and enzymatic turnover of pectin. The pectin methylesterases (PMEs), responsible for de-esterification, encompass a protein family of more than 60 isoforms in the Arabidopsis genome. The pivotal role of PME in the regulation of pectin properties also requires tight control at the post-translational level. Type-I PMEs are characterized by an N-terminal pro region, which exhibits homology with pectin methylesterase inhibitors (PMEIs). Here, we demonstrate that the proteolytic removal of the N-terminal pro region depends on conserved basic tetrad motifs, occurs in the early secretory pathway, and is required for the subsequent export of the PME core domain to the cell wall. In addition, we demonstrate the involvement of AtS1P, a subtilisin-like protease, in Arabidopsis PME processing. Our results indicate that the pro region operates as an effective retention mechanism, keeping unprocessed PME in the Golgi apparatus. Consequently, pro-protein processing ...Continue Reading

References

Oct 5, 1990·European Journal of Biochemistry·C BalestrieriL Servillo
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S Brown, J L Goldstein
Sep 11, 2001·The Plant Cell·B A PhillipsonJ Denecke
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·O LenzW Garten
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert E CampbellRoger Y Tsien
Jul 30, 2003·Journal of Virology·Martin J VincentStuart T Nichol
Dec 4, 2003·Acta Crystallographica. Section D, Biological Crystallography·Michael HothornKlaus Scheffzek
Dec 17, 2003·FEBS Letters·Sebastian WolfSteffen Greiner
Mar 8, 2005·Trends in Plant Science·Mansour KarimiPierre Hilson
Jun 14, 2005·Plant Physiology·Maurice BoschPeter K Hepler
Mar 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jason D SterlingDebra Mohnen
Mar 28, 2006·Developmental Biology·Guo-Wei TianVitaly Citovsky
May 20, 2006·FEBS Letters·Yuri L DorokhovJoseph G Atabekov
Sep 19, 2006·Plant Physiology·Kirk E FrancisGregory P Copenhaver
May 15, 2007·Trends in Plant Science·Jérôme PellouxEwa J Mellerowicz
Jul 31, 2007·The Plant Journal : for Cell and Molecular Biology·Jian-Xiang LiuStephen H Howell
Aug 2, 2007·The Plant Journal : for Cell and Molecular Biology·Brook K NelsonAndreas Nebenführ

❮ Previous
Next ❯

Citations

May 7, 2011·Cell Death and Differentiation·A B VartapetianT J Wolpert
Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Melani A AtmodjoDebra Mohnen
May 9, 2012·PloS One·Yuri L DorokhovYuri Y Gleba
Jul 25, 2014·Journal of Experimental Botany·Fabien SénéchalJérôme Pelloux
Mar 5, 2013·Annual Review of Plant Biology·Melani A AtmodjoDebra Mohnen
Jan 10, 2012·Annual Review of Plant Biology·Sebastian WolfHerman Höfte
Jul 11, 2013·Plant Reproduction·María Dolores GómezLuis A Cañas
Apr 1, 2015·Plant Signaling & Behavior·Fabien SénéchalJérôme Pelloux
Oct 20, 2011·Physiologia Plantarum·Liana TsiatsianiFrank Van Breusegem
Oct 15, 2009·Molecular Plant·Sebastian WolfJérome Pelloux
Feb 23, 2010·Plant Signaling & Behavior·Rolf RoseChristian Ottmann
Jun 28, 2011·Plant Signaling & Behavior·Monica De CaroliGabriella Piro
Jun 15, 2014·Protein Expression and Purification·Stephan PlattnerHolger Bohlmann
Jun 5, 2016·Journal of Experimental Botany·Michael MeyerAndreas Schaller
Dec 4, 2013·Journal of Proteome Research·Mikko T LehtonenJari P T Valkonen
Mar 7, 2017·Nature Plants·Henrik V Scheller
May 4, 2017·The New Phytologist·Andreas SchallerPablo Vera
Mar 23, 2018·International Journal of Molecular Sciences·Mateusz Majda, Stéphanie Robert
Dec 20, 2018·Journal of Integrative Plant Biology·Hiroyuki Nonogaki
May 28, 2015·The Journal of Biological Chemistry·Stephan PlattnerHolger Bohlmann
Sep 1, 2020·Plant Reproduction·Yongxian LuMatthew M S Evans
Oct 6, 2019·Scientific Reports·Daniela PontiggiaBenedetta Mattei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.