PMID: 7539921Jun 6, 1995Paper

The N-terminal region (A/B) of rat thyroid hormone receptors alpha 1, beta 1, but not beta 2 contains a strong thyroid hormone-dependent transactivation function

Proceedings of the National Academy of Sciences of the United States of America
H TomuraV M Nikodem

Abstract

In this study we have investigated the role of the N-terminal region of thyroid hormone receptors (TRs) in thyroid hormone (TH)-dependent transactivation of a thymidine kinase promoter containing TH response elements composed either of a direct repeat or an inverted palindrome. Comparison of rat TR beta 1 with TR beta 2 provides an excellent model since they share identical sequences except for their N termini. Our results show that TR beta 2 is an inefficient TH-dependent transcriptional activator. The degree of transactivation corresponds to that observed for the mutant TR delta N beta 1/2, which contains only those sequences common to TR beta 1 and TR beta 2. Thus, TH-dependent activation appears to be associated with two separate domains. The more important region, however, is embedded in the N-terminal domain. Furthermore, the transactivating property of TR alpha 1 was also localized to the N-terminal domain between amino acids 19 and 30. Using a coimmunoprecipitation assay, we show that the differential interaction of the N terminus of TR beta 1 and TR beta 2 with transcription factor IIB correlates with the TR beta 1 activation function. Hence, our results underscore the importance of the N-terminal region of TRs in TH-d...Continue Reading

References

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Citations

Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·J D BaxterT S Scanlan
Jul 21, 2012·The Journal of Biological Chemistry·Manohara S MavinakereLizabeth A Allison
Nov 11, 1998·Clinical and Experimental Pharmacology & Physiology. Supplement·J W AprilettiJ D Baxter
Feb 5, 2004·Journal of Endocrinological Investigation·A CrescenziM D'Armiento
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·L A CroftsJ A Eisman
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marta KostrouchovaJoseph Edward Rall
Sep 20, 1997·The Journal of Biological Chemistry·J R Wilkinson, H C Towle
Dec 31, 1997·The Journal of Biological Chemistry·T MondenA N Hollenberg
Jan 6, 2017·The Journal of Endocrinology·Gabriela Hernández-PugaAurea Orozco
Feb 15, 2018·The Journal of Endocrinology·Cyril S Anyetei-AnumLizabeth A Allison
Jun 29, 2002·Molecular Endocrinology·Hajer GuissoumaBarbara A Demeneix
Jun 9, 2017·FEBS Open Bio·Elias J FernandezJacob Amburn
Jun 28, 2001·Physiological Reviews·P M Yen
Oct 31, 2017·Pharmacology & Therapeutics·Elias J Fernandez
Oct 3, 2002·Journal of Cell Science·Joseph A NielsenRegina C Armstrong

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