The N-terminal segment of glyceraldehyde-3-phosphate dehydrogenase of Haemonchus contortus interacts with complements C1q and C3

Parasite Immunology
G V VedamurthyP Joshi

Abstract

Haemonchus contortus, an economically important blood-sucking parasite of sheep and goats, survives the harsh host gut environment by secreting a number of proteins referred as excretory/secretory (ES) products. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme, is one of the components of H. contortus ES products. The parasite enzyme binds to complement C3 and inhibits its activity. In this study, the C3-binding activity of the parasite GAPDH was mapped to the N-terminal part of the enzyme by generating defined recombinant fragments of the protein. The N-terminal fragment also trapped complement C1q but not C5 and inhibited complement-mediated lysis of sensitized sheep erythrocytes. Competitive binding assay indicates different binding regions for C1q and C3 proteins. GAPDH stimulated proliferation of goat peripheral blood mononuclear cells in vitro and reacted with the sera from H. contortus-infected animals. However, the fragments of GAPDH did not stimulate cell proliferation nor reacted with the infected animal sera. Furthermore, denatured GAPDH failed to react with the infected animal sera in dot blot suggesting conformation-dependent epitope. These results demonstrate an elegant strategy of the parasit...Continue Reading

References

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Feb 11, 1998·Clinical Microbiology Reviews·J P MartínezW L Chaffin
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Jan 13, 2009·The Biochemical Journal·Marek MichalakMichal Opas
May 12, 2009·Molecular and Biochemical Parasitology·S NareshaP Joshi

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Citations

Dec 28, 2018·Parasites & Vectors·Zixia WangXinping Zhu
Apr 6, 2019·Frontiers in Microbiology·Shuai ShaoXinping Zhu
Oct 30, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·David PirovichPatrick J Skelly
Dec 15, 2018·Parasite Immunology·Parvathy RajanParitosh Joshi
Sep 30, 2021·PLoS Neglected Tropical Diseases·Lucienne TrittenYovany Moreno

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