The N terminus of pro-endothelial monocyte-activating polypeptide II (EMAP II) regulates its binding with the C terminus, arginyl-tRNA synthetase, and neurofilament light protein.

The Journal of Biological Chemistry
Haiming XuMargaret A Schwarz

Abstract

Pro-endothelial monocyte-activating polypeptide II (EMAP II), one component of the multi-aminoacyl tRNA synthetase complex, plays multiple roles in physiological and pathological processes of protein translation, signal transduction, immunity, lung development, and tumor growth. Recent studies have determined that pro-EMAP II has an essential role in maintaining axon integrity in central and peripheral neural systems where deletion of the C terminus of pro-EMAP II has been reported in a consanguineous Israeli Bedouin kindred suffering from Pelizaeus-Merzbacher-like disease. We hypothesized that the N terminus of pro-EMAP II has an important role in the regulation of protein-protein interactions. Using a GFP reporter system, we defined a putative leucine zipper in the N terminus of human pro-EMAP II protein (amino acid residues 1-70) that can form specific strip-like punctate structures. Through GFP punctum analysis, we uncovered that the pro-EMAP II C terminus (amino acids 147-312) can repress GFP punctum formation. Pulldown assays confirmed that the binding between the pro-EMAP II N terminus and its C terminus is mediated by a putative leucine zipper. Furthermore, the pro-EMAP II 1-70 amino acid region was identified as the bi...Continue Reading

References

Jun 8, 1999·The Journal of Biological Chemistry·S G ParkK Shiba
Apr 9, 2001·The Journal of Biological Chemistry·Y G KoS Kim
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·F R Zhang, M A Schwarz
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jin Young KimSunghoon Kim
Dec 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Margaret A SchwarzMatthew K Lee
Mar 24, 2004·Seminars in Cell & Developmental Biology·Christopher M Dobson
Feb 1, 2005·The American Journal of Pathology·Sang Gyu ParkSunghoon Kim
Apr 20, 2005·Advances in Protein Chemistry·Derek N Woolfson
Sep 2, 2006·Cytokine & Growth Factor Reviews·Remco van HorssenTimo L M ten Hagen
Sep 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sang Gyu ParkSunghoon Kim
May 2, 2008·Biochemical and Biophysical Research Communications·Yeon Sook LeeSunghoon Kim
Nov 13, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Niranjan AwasthiRoderich E Schwarz
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xiaodong ZhuJiawei Zhou
Jun 1, 2010·Biochemical and Biophysical Research Communications·Gyuyoup KimSunghoon Kim
Nov 26, 2010·American Journal of Human Genetics·Miora FeinsteinOhad S Birk
Mar 15, 2011·American Journal of Human Genetics·Roberta BiancheriMirella Filocamo
Sep 11, 2014·International Journal of Molecular Sciences·Guobin LiPeng Lun

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May 11, 2019·Nature Reviews. Drug Discovery·Nam Hoon KwonSunghoon Kim

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