PMID: 9420216Jan 7, 1998Paper

The N terminus of rotavirus VP2 is necessary for encapsidation of VP1 and VP3

Journal of Virology
C Q ZengJ Cohen

Abstract

The innermost core of rotavirus is composed of VP2, which forms a protein layer that surrounds the two minor proteins VP1 and VP3, and the genome of 11 segments of double-stranded RNA. This inner core layer surrounded by VP6, the major capsid protein, constitutes double-layered particles that are transcriptionally active. Each gene encoding a structural protein of double-layered particles has been cloned into baculovirus recombinants and expressed in insect cells. Previously, we showed that coexpression of different combinations of the structural proteins of rotavirus double-layered particles results in the formation of virus-like particles (VLPs), and each VLP containing VP1, the presumed RNA-dependent RNA polymerase, possesses replicase activity as assayed in an in vitro template-dependent assay system (C. Q.-Y. Zeng, M. J. Wentz, J. Cohen, M. E. Estes, and R. F. Ramig, J. Virol. 70:2736-2742, 1996). This work reports construction and characterization of VLPs containing a truncated VP2 (VPdelta2, containing amino acids [aa] Met-93 to 880). Expression of VPdelta2 alone resulted in the formation of single-layered delta2-VLPs. Coexpression of VPdelta2 with VP6 produced double-layered delta2/6-VLPs. VLPs formed by coexpression of...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 25, 1991·The New England Journal of Medicine·N R Blacklow, H B Greenberg
Feb 1, 1991·The Journal of General Virology·J L PizarroE Spencer
Jun 1, 1991·Journal of Virology·M LabbéJ Cohen
Jan 20, 1988·Journal of Molecular Biology·B V PrasadW Chiu
Dec 1, 1994·The Journal of General Virology·M LabbéJ Cohen
Jan 1, 1994·Current Topics in Microbiology and Immunology·B V Prasad, W Chiu
May 15, 1994·Virology·C Q ZengM K Estes

❮ Previous
Next ❯

Citations

Jan 15, 2003·Journal of Virology·Mabel BeroisJean Cohen
Mar 3, 2004·The Journal of General Virology·Catherine EichwaldOscar R Burrone
Apr 24, 2010·Journal of Biomedicine & Biotechnology·Naima G Cortes-PerezGermain Trugnan
Apr 19, 2013·The Journal of General Virology·Crystal E BoudreauxSarah M McDonald
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Catherine EichwaldOscar R Burrone
Mar 5, 2010·The Journal of General Virology·R ContinO R Burrone
Jan 21, 2011·Journal of Virology·Sarah M McDonald, John T Patton
Jan 10, 2020·Journal of Virology·Antonino ButtafuocoCatherine Eichwald
Mar 31, 2010·The Journal of Biological Chemistry·Kristen M GuglielmiJohn T Patton
Jan 9, 1999·Journal of Virology·J T Patton, D Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.