PMID: 2109634Feb 27, 1990Paper

The N2-guanine adduct but not the C8-guanine or N6-adenine adducts formed by 4-nitroquinoline 1-oxide blocks the 3'-5' exonuclease action of T4 DNA polymerase

Biochemistry
G B Panigrahi, I G Walker

Abstract

When O-acetyl-4-(hydroxyamino)quinoline 1-oxide (Ac-4HAQO) reacts with double-stranded DNA at 37 degrees C the major products, N2-guanine, C8-guanine, and N6-adenine adducts, are formed in the proportions of 5:3:2, respectively. When the reaction is carried out with single-stranded DNA at 0 degree C, the products are found in the ratio 1:7:2. Unique 174-bp DNA fragments were modified in these ways and used as substrates for the 3'-5' exonuclease activity of T4 DNA polymerase. The results obtained showed that the exonuclease is blocked by the N2-guanine adduct but not the other two adducts. Interpretation of the cleavage patterns suggested that the enzyme stopped 2 nucleotides before the N2-guanine adduct. The N2-guanine adduct lies in the minor groove of the DNA double helix, while the other two adducts are found in the major groove. Apparently, only the former hinders progression of the enzyme.

References

Jan 1, 1988·Annual Review of Biochemistry·A Sancar, G B Sancar
Nov 14, 1986·Biochemical and Biophysical Research Communications·G B Panigrahi, I G Walker
Jan 1, 1987·Journal of Cell Science. Supplement·A Collins, R T Johnson
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Citations

Jun 27, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Amethist S FinchSteven E Rokita
Jul 9, 2008·Medical Mycology·P P DwivediA Dongari-Bagtzoglou
Jul 11, 1990·Nucleic Acids Research·W B Mattes
Jan 25, 1991·Nucleic Acids Research·D C ThomasI G Walker
Nov 25, 1992·Nucleic Acids Research·G B PanigrahiP D Sadowski
Mar 26, 2003·Nucleic Acids Research·Soobong ParkNatalia Tretyakova
Jan 7, 2011·Cancer Prevention Research·Keisuke ShiraiToshihiko Kawamori
Sep 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Katerina StratiPaul F Lambert
Dec 7, 2007·Oral Oncology·E VairaktarisE Patsouris
Jan 6, 2009·Molecular Carcinogenesis·Guliang Wang, Karen M Vasquez
Mar 18, 1995·Chemico-biological Interactions·B Jollès, A Laigle
Jan 1, 2012·Cells·Paul J IppolitiPenny J Beuning
Jan 12, 2019·Molecular Carcinogenesis·Yan-Xia ZhouAntoine M Snijders
Apr 23, 2019·Journal of Dental Research·P J Polverini, M W Lingen
Jan 22, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xiao-Han TangLorraine J Gudas
May 25, 2016·Cancer Research·Daniele ViarisioMassimo Tommasino

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