The Na+ -coupled glucose transporter SGLT2 interacts with its accessory unit MAP17 in vitro and their expressions overlap in the renal proximal tubule

FEBS Letters
Joaquim CaladoJosé Ramalho

Abstract

Na+ -glucose cotransporter 2 is the renal Na+ -coupled glucose transporter responsible for the tubular glucose reabsorption, while MAP17 was recently identified as its accessory unit. Mutations in either of the proteins' coding genes, SLC5A2 and PDZK1IP1, lead to urinary glucose excretion. To investigate whether MAP17 interacts with SGLT2 in vitro, we engineered a V5-tagged SGLT2 construct and evaluated HEK293T cells coexpressing it together with a HA tagged MAP17 construct. MAP17 is shown to colocalize and coimmunoprecipitate with SGLT2. Also, in human kidney sections, the expression of both proteins overlaps at the apical surface of tubular epithelia. This interaction provides the rationale behind SGLT2 activation by MAP17 as well the similarity of the SLC5A2 and PDZK1IP1 glucosuric phenotypes.

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A HedigerE M Wright
Jan 31, 2003·Molecular and Cellular Biology·Olivier KocherAnnalyn Gilchrist
Jul 3, 2003·American Journal of Physiology. Renal Physiology·Sandra PribanicHeini Murer
Oct 9, 2003·Kidney International·Serge M GislerHeini Murer
Oct 22, 2003·Journal of the American Society of Nephrology : JASN·René SanterDan Klaerke
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Carlos M FarinhaMargarida D Amaral
Aug 24, 2006·American Journal of Physiology. Renal Physiology·Miguel A LanaspaVíctor Sorribas
Jul 10, 2010·Journal of the American Society of Nephrology : JASN·Volker VallonTimo Rieg
Oct 29, 2010·American Journal of Physiology. Cell Physiology·Charles S HummelErnest M Wright
Mar 5, 2011·Kidney International. Supplement·Joaquim CaladoJosé Rueff
Apr 30, 2011·Physiological Reviews·Ernest M WrightBruce A Hirayama
Jan 21, 2012·American Journal of Physiology. Cell Physiology·Ivan SabolicHermann Koepsell
Sep 18, 2015·Journal of the American Society of Nephrology : JASN·Nasser DhayatDaniel G Fuster
Mar 5, 2016·Journal of Cell Science·Sílvia Vale-CostaMaria João Amorim
Jun 12, 2016·Journal of the American Society of Nephrology : JASN·Michael J CoadyJean-Yves Lapointe
Apr 19, 2017·Diabetes, Obesity & Metabolism·Anna SoliniEle Ferrannini
Jun 9, 2017·American Journal of Physiology. Renal Physiology·Michael J CoadyJean-Yves Lapointe

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Citations

Mar 19, 2021·Frontiers in Pharmacology·Giuliano Ciarimboli
Jun 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yuping ZhangArul M Chinnaiyan

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