The NAC family transcription factor GmNAC42-1 regulates biosynthesis of the anticancer and neuroprotective glyceollins in soybean

BMC Genomics
Md Asraful JahanNik Kovinich

Abstract

Glyceollins are isoflavonoid-derived pathogen-inducible defense metabolites (phytoalexins) from soybean (Glycine max L. Merr) that have important roles in providing defense against pathogens. They also have impressive anticancer and neuroprotective activities in mammals. Despite their potential usefulness as therapeutics, glyceollins are not economical to synthesize and are biosynthesized only transiently and in low amounts in response to specific stresses. Engineering the regulation of glyceollin biosynthesis may be a promising approach to enhance their bioproduction, yet the transcription factors (TFs) that regulate their biosynthesis have remained elusive. To address this, we first aimed to identify novel abiotic stresses that enhance or suppress the elicitation of glyceollins and then used a comparative transcriptomics approach to search for TF gene candidates that may positively regulate glyceollin biosynthesis. Acidity stress (pH 3.0 medium) and dehydration exerted prolonged (week-long) inductive or suppressive effects on glyceollin biosynthesis, respectively. RNA-seq found that all known biosynthetic genes were oppositely regulated by acidity stress and dehydration, but known isoflavonoid TFs were not. Systemic acquired ...Continue Reading

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Citations

Dec 30, 2020·Journal of Biotechnology·Sajjad AhmedNik Kovinich
Dec 31, 2020·International Journal of Molecular Sciences·Zhiyuan BianChongying Wang
Jun 25, 2021·Frontiers in Plant Science·Soo In SohnYoun Sung Cho

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Datasets Mentioned

BETA
AT2G43000

Methods Mentioned

BETA
transgenic
RNA-seq
Chip
PCR
fluorescence
one-hybrid

Software Mentioned

MEGA
Muscle
FastQC
NIS Elements
STAR RNA - seq aligner
DESeq2

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