The Na+,K+-ATPase carrying the carboxy-terminal Ca2+/calmodulin binding domain of the Ca2+ pump has 2Na+,2K+ stoichiometry and lost charge movement in Na+/Na+ exchange

FEBS Letters
S H YoshimuraW Schwarz

Abstract

An altered ion-transport stoichiometry from 3Na+,2K+ to 2Na+,2K+ is observed in a chimeric Na+,K+ATPase, which carries the Ca2+/calmodulin binding domain (CBD) of the plasma membrane Ca2+-ATPase at its carboxy-terminus [Zhao et al., FEBS Lett. 408 (1997) 271-2751. The ouabain-resistant mutant of this chimera (ORalpha1-CBD) was constructed to further investigate the effect of the CBD on ion-transport properties. The ORalpha1-CBD still shows the 2Na+,2K+ stoichiometry. The loss of electrogenicity is accompanied by the disappearance of transient charge movements in the Na+/Na+ exchange mode. We conclude that the binding of the third Na+ ion, but not of the two others, in 3Na+,2K+ transport mode apparently senses the electric field, and that the voltage-dependent Na+ binding is likely to be lost in the chimera with CBD.

References

Dec 10, 1987·FEBS Letters·S NoguchiS Numa
Jan 1, 1986·The Journal of Membrane Biology·A V Lafaire, W Schwarz
Jan 1, 1993·The Journal of General Physiology·R F Rakowski
Jan 15, 1997·The Journal of Membrane Biology·R F RakowskiP De Weer

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Citations

May 24, 2003·Annals of the New York Academy of Sciences·Shige H Yoshimura, Kunio Takeyasu
Jun 5, 2008·Journal of Cell Science·Shige H YoshimuraKunio Takeyasu
Apr 22, 2005·Biochimica Et Biophysica Acta·Jan B KoenderinkThomas Friedrich

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