The Nanomechanics of Lipid Multibilayer Stacks Exhibits Complex Dynamics

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Josep Relat-GobernaSergi Garcia-Manyes

Abstract

The nanomechanics of lipid membranes regulates a large number of cellular functions. However, the molecular mechanisms underlying the plastic rupture of individual bilayers remain elusive. This study uses force clamp spectroscopy to capture the force-dependent dynamics of membrane failure on a model diphytanoylphosphatidylcholine multilayer stack, which is devoid of surface effects. The obtained kinetic measurements demonstrate that the rupture of an individual lipid bilayer, occurring in the bilayer parallel plane, is a stochastic process that follows a log-normal distribution, compatible with a pore formation mechanism. Furthermore, the vertical individual force-clamp trajectories, occurring in the bilayer orthogonal bilayer plane, reveal that rupturing process occurs through distinct intermediate mechanical transition states that can be ascribed to the fine chemical composition of the hydrated phospholipid moiety. Altogether, these results provide a first description of unanticipated complexity in the energy landscape governing the mechanically induced bilayer rupture process.

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Citations

Jan 29, 2021·The Journal of Physical Chemistry. B·Mason L ValentineCarlos R Baiz
Nov 10, 2020·ACS Omega·Frédéric F Harb, Bernard Tinland
May 25, 2021·Frontiers in Molecular Biosciences·Massimiliano GalluzziJiangyu Li
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Jun 14, 2018·The Journal of Physical Chemistry. B·Florian FranzFrauke Gräter
Jul 10, 2019·ACS Applied Materials & Interfaces·Vytautas NavikasRamu Nas Valiokas

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