The native structure of annexin A2 peptides in hydrophilic environment determines their anti-angiogenic effects

Biochemical Pharmacology
Aase M RaddumAnni Vedeler

Abstract

The progression of aggressive cancer occurs via angiogenesis and metastasis makes these processes important targets for the development of anti-cancer agents. However, recent studies have raised the concern that selective inhibition of angiogenesis results in a switch towards increased tumour growth and metastasis. Since Annexin A2 (AnxA2) is involved in both angiogenesis and metastasis, it may serve as an ideal target for the simultaneous inhibition of both processes. Based on the discovery that domains I (D(I)) and IV (D(IV)) of AnxA2 are potent inhibitors of angiogenesis, we designed seven peptides derived from these domains based on AnxA2 crystal structures. The peptides were expressed as fusion peptides to increase their folding and solubility. Light scattering, far-UV circular dichroism and thermal transition analyses were employed to investigate their aggregation tendencies, α-helical propensity and stability, respectively. 2,2,2-trifluoroethanol (50%) increased the α-helical propensities of all peptides, indicating that they may favour a hydrophobic environment, but did not enhance their thermal stability. D(I)-P2 appears to be the most stable and folded peptide in a hydrophilic environment. The secondary structure of D...Continue Reading

References

Aug 13, 1996·Biochemistry·F Cordier-OchsenbeinA Sanson
Apr 21, 1999·QJM : Monthly Journal of the Association of Physicians·C M EdwardsS R Bloom
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Jun 7, 2000·The Biochemical Journal·A Vedeler, H Hollås
Nov 9, 2000·The Journal of Biological Chemistry·N R Filipenko, D M Waisman
Sep 26, 2001·Protein Expression and Purification·Y NominéG Travé
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Aug 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Danilo RoccatanoAlan E Mark
Jun 5, 2003·Nature Reviews. Cancer·Gabriele Bergers, Laura E Benjamin
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Dec 31, 2003·The Journal of Cell Biology·Christelle BenaudJacques Baudier
Jan 1, 2004·The Journal of Clinical Investigation·Qi LingKatherine A Hajjar
Jun 1, 2004·Journal of Cell Science·Ursula Rescher, Volker Gerke
Oct 20, 2004·Journal of Cellular Physiology·Benoît J GentilJacques Baudier
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Nov 23, 2005·Oncology·Peter Carmeliet
Dec 16, 2005·Nature·Peter Carmeliet
Apr 8, 2006·The EMBO Journal·Matthew J HayesStephen E Moss
Jul 21, 2006·Acta Crystallographica. Section D, Biological Crystallography·Wladek MinorMaksymilian Chruszcz
Aug 3, 2006·The Journal of Biological Chemistry·Chenghua ShaoBarbara A Seaton
Oct 10, 2006·The AAPS Journal·Yingjuan LuEmanuela Sega
Oct 10, 2006·The AAPS Journal·Mary E M CromwellFred Jacobson
May 25, 2007·Nature Reviews. Molecular Cell Biology·Ralf H Adams, Kari Alitalo
Dec 28, 2007·Molecular and Cellular Biology·Stéphanie HeyraudDanielle Gulino-Debrac
Jan 29, 2008·Current Pharmaceutical Design·Mahesh C Sharma, Meena Sharma
May 23, 2009·British Journal of Pharmacology·Patrick ChamesDaniel Baty
Dec 19, 2009·The Journal of Biological Chemistry·Kamala KesavanDouglas B Jacoby
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Apr 13, 2011·Trends in Molecular Medicine·Pipsa SaharinenKari Alitalo
Apr 14, 2011·Journal of Cell Science·Mallika ValapalaJamboor K Vishwanatha
Sep 20, 2011·Cell·Michael PotentePeter Carmeliet
Apr 17, 2012·International Journal of Cell Biology·Adam G GrieveMatthew J Hayes
Jun 20, 2012·Current Protein & Peptide Science·Anni VedelerAase M Raddum
Oct 24, 2012·Biological Chemistry·Gabriel OzorowskiHartmut Luecke
Nov 24, 2012·Biochimica Et Biophysica Acta·Sara Marie Øie SolbakTorgils Fossen
Jul 11, 2013·World Journal of Gastroenterology : WJG·Hai-Jian ZhangShao-Lin Lu

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