The natural history of insulin content in the pancreas of female and male non-obese diabetic mouse: implications for trials of diabetes prevention in humans

Diabetes/metabolism Research and Reviews
M G ValoraniP Pozzilli

Abstract

The non-obese diabetic (NOD) mouse is a well-established animal model used to study the pathogenesis of type 1 diabetes. The NOD mouse spontaneously develops an autoimmune form of the disease between 12 and 18 weeks of age, characterized by an infiltration of the endocrine pancreas by autoreactive mononuclear cells. In our colony, all animals showed signs of insulitis, but only approximately 60% of females and 15% of males developed diabetes. The aim of this study was to determine the natural history of insulin content in the pancreas of female and male NOD/Ba mice during their life span. Pancreata were collected at two-week intervals, from 4 weeks of age to 30 weeks of age. Four animals at each age as well as 18 diabetic female NOD mice were studied. Pancreata were homogenized, supernatants collected and insulin measured by radioimmunoassay. Female and male non-diabetic NOD mice showed significantly higher levels of insulin in the pancreata in comparison to the diabetic female animals. Pancreata from female (n = 56) animals showed more insulin content than male pancreata (n = 56), suggesting beta-cell hyperactivity as a result of the ongoing beta-cell destruction. However this difference was only significant at an early age (4...Continue Reading

Citations

Oct 1, 2016·Molecular Metabolism·Zulaykho ShamansurovaJulie L Lavoie
Jun 17, 2005·Diabetes/metabolism Research and Reviews

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Y W Kan, A M Dozy
Proceedings of the National Academy of Sciences of the United States of America
D F Steiner, P E Oyer
Proceedings of the National Academy of Sciences of the United States of America
S J ChanD Steiner
The Journal of Clinical Endocrinology and Metabolism
M A PermuttR Chyn
© 2021 Meta ULC. All rights reserved