PMID: 1200985Jun 1, 1975Paper

The nature and control of the tricarboxylate cycle in beetle flight muscle

The Biochemical Journal
R G Hansford, R N Johnson

Abstract

The only exogenous substrates oxidized by mitochondria isolated from the flight muscle of the Japanese beetle (Popillia japonica) are proline, pyruvate and glycerol 3-phosphate. The highest rate of oxygen consumption is obtained with proline. The oxidation of proline leads to the production of more NH3 than alanine, indicating a functioning glutamate dehydrogenase (EC 1.4.1.2). Studies of mitochondrial extracts confirm the presence of a very active glutamate dehydrogenase, and this enzyme is found to be activated by ADP and inhibited by ATP. These extracts also show high alanine aminotransferase activity (EC 2.6.1.2) and a uniquely active "malic' enzyme (EC 1.1.1.39). The "malic' enzyme is activated by succinate and inhibited by ATP and by pyruvate. It is suggested that the input of tricarboxylate-cycle intermediate from proline oxidation is balanced by the formation of pyruvate from malate, and the complete oxidation of the majority of the pyruvate. Studies of the steady-state concentrations of mitochondrial CoASH and CoA thioesters during proline oxidation show a high succinyl (3-carboxypropionyl)-CoA content which falls on activating respiration with ADP. There is a concomitant rise in CoASH. However, the reverse transition,...Continue Reading

Citations

Dec 1, 1987·Archives internationales de physiologie et de biochimie·J K Teller
Mar 21, 2006·Molecular Genetics and Metabolism·Julian A Martinez Agosto, Edward R B McCabe
Mar 1, 1988·Fish Physiology and Biochemistry·K B Storey, J H Fields
Feb 1, 1982·Paraplegia·G Bedbrook
Jul 1, 2008·The Biochemical Journal·Cecilia GiuliviShirley Luckhart
Apr 15, 1983·Biochimica Et Biophysica Acta·R G Hansford
Mar 14, 1980·Biochimica Et Biophysica Acta·J SwierczyńskiZ Aleksandrowicz
Jan 1, 1978·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R G Hansford
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R G Hansford, R N Johnson
Jun 14, 2003·Journal of Insect Physiology·P Y Scaraffia, M A Wells
Sep 27, 2003·Journal of Insect Physiology·Shuji ItakuraMichael Slaytor

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