The nature of membrane anchorage determines kinase association and detergent solubility of CD4

Biochemical and Biophysical Research Communications
K R SolomonR W Finberg

Abstract

The presence of a glycosylphosphatidylinositol (GPI) anchor on a membrane protein is thought to influence aspects of the protein's biochemistry. While it has been demonstrated that a GPI-anchor is sufficient for altering the detergent solubility of integral membrane proteins, it has not been shown that the anchor is sufficient for changing the phosphoprotein associations of membrane proteins. In order to define the influence of GPI-anchors on the biochemistry of membrane proteins we compared the phosphoprotein associations and detergent solubility of wild-type and GPI-anchored CD4 expressed on HSB cell transfectants. While wild-type CD4 was mostly associated with lck kinase, GPI-anchored CD4 was associated with the 'GPI-anchored pattern of phosphoproteins'. The Triton X-100 solubilities of the two forms of CD4 were also distinct: wild-type CD4 was > 95% soluble, whereas GPI-anchored CD4 was only 65% soluble. These results underscore the deterministic role of the GPI-anchor in the properties associated with GPI-anchored proteins.

References

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Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·T E Thompson, T W Tillack
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Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·K R SolomonR W Finberg

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Citations

Nov 15, 2006·The Journal of Cell Biology·Thomas B Nicholson, Clifford P Stanners
Mar 14, 2007·Biochemical and Biophysical Research Communications·Rick F ThorneGordon F Burns
Oct 21, 2006·Biochemical and Biophysical Research Communications·Rick F ThorneGordon F Burns
Dec 29, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K R SolomonP V Hauschka
Dec 29, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K R SolomonP V Hauschka
Jun 6, 2009·The American Journal of Pathology·Christian SundbergKeith R Solomon

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