Abstract
We report in this study that the nature of the injected immunogen or infection of mice can change the specificity of antibodies against different epitopes of the mycobacterial 38 kDa antigen. Following immunization with a selection of 14 peptides, only p65-83 and p285-304 resulted in antibodies binding to the whole antigen, whereas the recombinant antigen induced antibodies to p201-220 and p230-249, which are non-immunogenic following peptide immunization. Killed tubercle bacilli stimulated antibodies exclusively against the N-terminal 1-20 peptide, whilst infection with live tubercle bacilli led to antibodies recognizing non-continuous epitopes, demonstrable by binding to the 38 kDa protein, but to none of the tested peptides. The most striking finding is that protein immunization failed to induce antibodies to p65-83, although this epitope is immunodominant for T cells, irrespective of the mode of sensitization. Since this peptide contains non-overlapping B (69-75) and T (75-81) cell stimulatory cores, it appears that T help dedicated to the adjacent B epitope following peptide immunization may have shifted in favour of B cells reacting either with distantly located linear epitopes p1-20 and p201-220 following immunization wi...Continue Reading
Citations
Nov 1, 1995·Vaccine·H M VordermeierM Singh
Mar 1, 1997·Molecular Immunology·D P HarrisJ Ivanyi
Jul 31, 1998·Molecular Immunology·K A WilkinsonF Hudecz
Aug 5, 2000·Vaccine·D P da FonsecaA F Verheul
Apr 27, 1999·Vaccine·N VenkataprasadH M Vordermeier
Jul 1, 1996·Immunology·D P HarrisJ Ivanyi
Feb 26, 2000·Infection and Immunity·P LefèvreK Huygen
Jul 12, 2001·Infection and Immunity·D P FonsecaA F Verheul
Nov 26, 2003·Infection and Immunity·Kai SchulzeCarlos A Guzmán
Apr 20, 2006·Infection and Immunity·Saet-Byel JungEun-Kyeong Jo
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Guglielmo LuccheseDarja Kanduc
Sep 21, 2011·Tuberculosis·Juraj Ivanyi
Apr 10, 2014·Frontiers in Immunology·Juraj Ivanyi
Feb 20, 2016·Journal of Immunological Methods·Ramgopal R MettuSamuel J Landry
Feb 18, 2003·Scandinavian Journal of Immunology·S TollefsenH G Wiker
Nov 26, 2003·Critical Reviews in Microbiology·Himanshu KumarR N K Bamezai
Oct 6, 2017·Oncotarget·Bin LiChao Wu
Jan 21, 2019·Microbial Cell Factories·Giroshi Bando-CamposNorma A Valdez-Cruz
Jun 25, 1998·Infection and Immunity·D P da FonsecaA F Verheul
Jul 5, 2003·Structure·Nand K VyasFlorante A Quiocho