PMID: 8951687Jan 1, 1996Paper

The nature of the inhibition of camel retina acetylcholinesterase (EC 3.1.1.7) activity by tetrahydroaminoacridine

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
A A al-Jafari

Abstract

The nature of the inhibition of camel retina acetylcholinesterase (AChE) activity by tetrahydro-aminoacridine (THA, tacrine) has been investigated in the present study. The non-significant change of the percent inhibition of AChE by THA with respect to various lengths of the preincubation period showed the type of the reversible inhibition. THA reversibly inhibited AChE activity in a concentration dependent manner; IC50 was 0.23 microM while the IC100 was 14.22 microM. The K(m) for the hydrolysis of acetylthiocholine iodide was found to be 62.6 microM in the control system; a value increased in the THA treated systems. The Vmax was 0.472 mumole/min/mg protein for the control system, while it decreased in the THA treated systems. Dixon, as well as Lineweaver-Burk, plots and their secondary replots indicated that the nature of the inhibition is of the linear mixed type, which is considered to be a partial competitive and pure non-competitive mixture. The values of Ki(slope) and K'i(intercept) were estimated as 0.068 microM and 0.181 microM, respectively. The K'i was greater than Ki indicating that THA has a greater affinity of binding for the peripheral site than the active site of the camel retina AChE. The use of camel retina a...Continue Reading

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Citations

Feb 1, 1997·Journal of Enzyme Inhibition·A A al-JafariA S Alhomida
Jul 25, 2006·Chemico-biological Interactions·Mushtaq AhmedMaria R C Schetinger
Jan 16, 1999·Journal of Biochemical and Molecular Toxicology·M A Kamal, A A Al-jafari
Aug 4, 2021·ChemMedChem·Karen G JoãoPaula B Andrade

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