The necessity of mitochondrial genome DNA for normal development of Dictyostelium cells

Journal of Cell Science
Junji ChidaYasuo Maeda

Abstract

Most unexpectedly, there is now increasing evidence that mitochondria have novel and crucial functions in the regulatory machinery of the growth/differentiation transition, cell-type determination, cellular movement and pattern formation. Here we created rho delta cells with a reduced amount (about 1/4) of mitochondrial DNA (mtDNA) from Dictyostelium discoideum Ax-2 cells, by exposing Ax-2 cells to ca. 30 microg/ml of ethidium bromide (EtBr) in axenic growth medium. Importantly, the rho delta cells exhibited a series of fascinating behaviors: when they were starved, they showed a marked delay of differentiation and stopped their development at the slug stage, thus failing to construct fruiting bodies. Moreover, cell patterning and cell-type proportioning were found to be greatly modified in slugs (referred to as rho delta slugs) derived from rho delta cells. That is, prestalk differentiation was significantly enhanced in rho delta slugs, while prespore differentiation was markedly inhibited. In addition, the clear anterior prestalk/posterior prespore pattern was considerably disturbed in rho delta slugs, presumably because of incomplete sorting between the two types of differentiated cells. After the assay of phototaxis, rho de...Continue Reading

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Citations

Apr 24, 2009·Molecular and Cellular Biochemistry·Sarah J Annesley, Paul R Fisher
Mar 3, 2007·Molecular Biology of the Cell·Paul B BokkoPaul R Fisher
Aug 1, 2009·Development, Growth & Differentiation·Kazutaka IshiiYasuo Maeda
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Mar 4, 2020·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Haruka HiraokaYasushi Hiraoka
Apr 22, 2010·Journal of Cell Science·Sergio Carilla-LatorreRicardo Escalante

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