PMID: 9531486Jun 11, 1998Paper

The negative charge of glutamic acid-820 in the gastric H+,K+-ATPase alpha-subunit is essential for K+ activation of the enzyme activity

The Biochemical Journal
H P HermsenJ J De Pont

Abstract

To investigate the role of Glu820, located in transmembrane domain M6 of the alpha-subunit of gastric H+,K+-ATPase, a number of mutants was prepared and expressed in Sf9 cells using a baculovirus encoding for both H+,K+-ATPase subunits. The wild-type enzyme and the E820D (Glu820-->Asp) mutant showed a similar biphasic activation by K+ on the ATPase activity (maximum at 1 mM). The mutant E820A had a markedly decreased K+ affinity (maximum at 40-100 mM). The other mutants, E820Q, E820N, E820L and E820K, showed no K+-activated ATPase activity at all, whereas all mutants formed a phosphorylated intermediate. After preincubation with K+ before phosphorylation mutant E820D showed a similar K+-sensitivity as the wild-type enzyme. The mutants E820N and E820Q had a 10-20 times lower sensitivity, whereas the other three mutants were hardly sensitive towards K+. Upon preincubation with 3-(cyanomethyl)-2-methyl-8-(phenylmethoxy) imidazo [1,2a]-pyridine (SCH28080), all mutants showed similar sensitivity for this drug as the wild-type enzyme, except mutant E820Q, which could only partly be inhibited, and mutant E820K, which was completely insensitive towards SCH28080. These experiments suggest that, with a relatively large residue at positio...Continue Reading

Citations

Mar 23, 2002·The Journal of Biological Chemistry·Tohru KimuraShinji Asano
Nov 25, 2003·The EMBO Journal·David G WinklerJohn A Latham
Jul 31, 2009·American Journal of Physiology. Renal Physiology·Michelle L GumzCharles S Wingo
Jun 9, 2001·The Journal of Biological Chemistry·S AsanoN Takeguchi
Dec 31, 2003·The Journal of Biological Chemistry·Shinji AsanoMagotoshi Morii
Jul 28, 2005·The Journal of Biological Chemistry·Herman G P SwartsJan Joep H H M De Pont
May 24, 2003·Annals of the New York Academy of Sciences·Jan Joep H H M De PontJan B Koenderink
Feb 6, 2004·The Journal of Biological Chemistry·Jan B KoenderinkJan Joep H H M De Pont
Nov 18, 2000·The Japanese Journal of Physiology·S AsanoN Takeguchi
Jan 13, 2005·The Journal of Biological Chemistry·Herman G P SwartsJan Joep H H M De Pont
May 23, 2001·Archives of Biochemistry and Biophysics·S J RulliE C Rabon
Sep 18, 2002·Archives of Biochemistry and Biophysics·Joan H F Drosopoulos
Jun 5, 2004·Biochemical and Biophysical Research Communications·P Hima BinduG Narahari Sastry
Dec 2, 2006·Biochimica Et Biophysica Acta·Herman G P SwartsJan Joep H H M De Pont
Jun 19, 2007·Biochimica Et Biophysica Acta·Guadalupe GuerraCarolyn W Slayman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.