The Netrin-related domain of Sfrp1 interacts with Wnt ligands and antagonizes their activity in the anterior neural plate.

Neural Development
Javier Lopez-RiosPaola Bovolenta

Abstract

Secreted frizzled related proteins (SFRPs) are multifunctional modulators of Wnt and BMP (Bone Morphogenetic Protein) signalling necessary for the development of most organs and the homeostasis of different adult tissues. SFRPs fold in two independent domains: the cysteine rich domain (SfrpCRD) related to the extracellular portion of Frizzled (Fz, Wnt receptors) and the Netrin module (SfrpNTR) defined by homologies with molecules such as Netrin-1, inhibitors of metalloproteinases and complement proteins. Due to its structural relationship with Fz, it is believed that SfrpCRD interferes with Wnt signalling by binding and sequestering the ligand. In contrast, the functional relevance of the SfrpNTR has been barely addressed. Here, we combine biochemical studies, mutational analysis and functional assays in cell culture and medaka-fish embryos to show that the Sfrp1NTR mimics the function of the entire molecule, binds to Wnt8 and antagonizes Wnt canonical signalling. This activity requires intact tertiary structure and is shared by the distantly related Netrin-1NTR. In contrast, the Sfrp1CRD cannot mirror the function of the entire molecule in vivo but interacts with Fz receptors and antagonizes Wnt8-mediated beta-catenin transcri...Continue Reading

References

Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·A RattnerJ Nathans
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·K LinF P Luyten
Oct 12, 2000·Mechanisms of Development·P EsteveP Bovolenta
Feb 13, 2001·Genes & Development·V A Schneider, M Mercola
Apr 5, 2003·Mechanisms of Development·Beate KilianCarl Philipp Heisenberg
May 2, 2003·Journal of Cell Science·Pilar EstevePaola Bovolenta
May 8, 2003·Journal of Cell Science·Clémence CarronMuriel Umbhauer
Sep 4, 2003·The Journal of Cell Biology·Trudi A WestfallDiane C Slusarski
Jun 24, 2004·Mechanisms of Development·Takashi Iwamatsu
Jun 24, 2004·Mechanisms of Development·Pilar EstevePaola Bovolenta
Oct 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chiann-Mun ChenGary Struhl
Sep 16, 2005·The EMBO Journal·Michael Povelones, Roel Nusse
Sep 21, 2005·Nature Neuroscience·Josana RodriguezPaola Bovolenta
Jan 18, 2006·Current Opinion in Neurobiology·Pilar Esteve, Paola Bovolenta
Jan 21, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lisa M GalliLaura W Burrus
Feb 17, 2006·Cellular Signalling·Hsien-yu WangCraig C Malbon
Mar 7, 2006·Nature Cell Biology·Osamu MuraokaMasahiko Hibi
Dec 13, 2006·The Journal of Biological Chemistry·Philip M SmallwoodJeremy Nathans
Apr 28, 2007·Biochemical and Biophysical Research Communications·Danuta WawrzakLuc Leyns
Mar 7, 2008·Journal of Cell Science·Paola BovolentaJavier Lopez-Rios
May 29, 2008·Doklady. Biochemistry and Biophysics·A E VoronkovN S Zefirov

❮ Previous
Next ❯

Citations

Sep 28, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kimberly A Mulligan, Benjamin N R Cheyette
Apr 12, 2011·Nature Neuroscience·Pilar EstevePaola Bovolenta
Jul 21, 2012·Genome Biology and Evolution·Lucas Leclère, Fabian Rentzsch
May 8, 2010·The Tohoku Journal of Experimental Medicine·Pilar Esteve, Paola Bovolenta
Dec 9, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Zhenzhen ZhaoXuerong Chen
Jun 10, 2009·Developmental Neurobiology·Jose Maria RuizPaola Bovolenta
Aug 26, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Cristina Sánchez-Camacho, Paola Bovolenta
Feb 24, 2011·European Journal of Haematology·Alexandra FilipovichKarl-Anton Kreuzer
Jan 25, 2014·Developmental Biology·Vanessa L HollyAndrew J Waskiewicz
Apr 12, 2016·The International Journal of Biochemistry & Cell Biology·Sudha WarrierArun Dharmarajan
Jan 13, 2009·Bone·Frank P LuytenRik J Lories
Nov 1, 2012·Mechanisms of Development·Leonardo BeccariPaola Bovolenta
Oct 2, 2013·Cellular Signalling·Charles P XavierJeffrey S Rubin
Dec 29, 2016·Nature Reviews. Immunology·Jan Felix, Savvas N Savvides
May 24, 2017·The Journal of Biological Chemistry·Mark AgostinoArun Dharmarajan
Oct 26, 2011·Nature Reviews. Urology·Sharanjot SainiRajvir Dahiya
Feb 23, 2020·Cancers·Rashidah BaharudinNurul Syakima Ab Mutalib
Jun 7, 2019·Nature Communications·Ilenia BernasconeFernando Martín-Belmonte
Jun 24, 2020·Disease Models & Mechanisms·Olga Medina-MartinezMilan Jamrich
Mar 22, 2020·Scientific Reports·Elsa CisnerosPaola Bovolenta
Jun 24, 2016·Molecular and Cellular Biochemistry·Archita Ghoshal, Siddhartha Sankar Ghosh
Mar 6, 2021·Journal of Biomolecular Structure & Dynamics·Raghava R SunkaraSanjeev K Waghmare
Nov 19, 2011·Development·Leonardo BeccariPaola Bovolenta

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
co-immunoprecipitation
pull-downs
glycosylation
immunoprecipitate
inmunoprecipitations
plasmon resonance
PCR
inmunoprecipitation
Transfection

Software Mentioned

SPSS
Leica

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.