The neuraminidase of influenza virus.

Proteins
G M Air, W Graeme Laver

Abstract

It is the enzyme neuraminidase, projecting from the surface of influenza virus particles, which allows the virus to leave infected cells and spread in the body. Antibodies which inhibit the enzyme limit the infection, but antigenic variation of the neuraminidase renders it ineffective in a vaccine. This article describes the crystal structure of influenza virus neuraminidase, information about the active site which may lead to development of specific and effective inhibitors of the enzyme, and the structure of epitopes (antigenic determinants) on the neuraminidase. The 3-dimensional structure of the epitopes was obtained by X-ray diffraction methods using crystals of neuraminidase complexed with monoclonal antibody Fab fragments. Escape mutants, selected by growing virus in the presence of monoclonal antibodies to the neuraminidase, possess single amino acid sequence changes. The crystal structure of two mutants showed that the change in structure was restricted to that particular sidechain, but the change in the epitope was sufficient to abolish antibody binding even though it is known in one case that 21 other amino acids on the neuraminidase are in contact with the antibody.

References

Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S SheriffD R Davies
May 1, 1988·Virology·R G WebsterW G Laver
Jan 1, 1989·Cold Spring Harbor Symposia on Quantitative Biology·W R TulipP M Colman
Jun 12, 1989·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P M ColmanR G Webster
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E A PadlanD R Davies
Sep 1, 1987·Journal of Virology·R G WebsterW G Laver
Apr 30, 1985·Virology·M C ElsW G Laver
Jan 1, 1984·Annual Review of Immunology·D C BenjaminE M Prager
Jan 1, 1983·Annual Review of Biochemistry·R A Lamb, P W Choppin
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J J SkehelD C Wiley
Mar 1, 1957·Biochimica Et Biophysica Acta·A GOTTSCHALK

❮ Previous
Next ❯

Citations

Jul 1, 1992·Medicinal Research Reviews·M D Walkinshaw
May 3, 2002·Reviews in Medical Virology·Ralf WagnerHans-Dieter Klenk
Sep 21, 2000·Folia Microbiologica·L Jindrák, L Grubhoffer
Oct 27, 2009·Journal of Molecular Modeling·Mohammed Noor Al-qattan, Mohd Nizam Mordi
Apr 1, 2006·Glycoconjugate Journal·Christel Schwegmann-Wessels, Georg Herrler
Mar 17, 2004·Bioorganic & Medicinal Chemistry·Kenji SasakiKazukiyo Kobayashi
Dec 13, 2002·Vaccine·Robert Mischler, Ian C Metcalfe
Jan 13, 2000·Bioorganic & Medicinal Chemistry·V R AtigaddaG W Laver
Nov 22, 2002·Microbes and Infection·Graeme Laver, Elspeth Garman
Aug 19, 2007·Animal Health Research Reviews·Gabriele A Landolt, Christopher W Olsen
Sep 18, 2012·Journal of Chemical Information and Modeling·Ariela Vergara-JaqueChristophe Chipot
May 19, 2011·Journal of Agricultural and Food Chemistry·Young Bae RyuWoo Song Lee
May 11, 2000·Pediatrics International : Official Journal of the Japan Pediatric Society·T Saito, M Tashiro
Dec 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Tokiko WatanabeYoshihiro Kawaoka
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xueyong ZhuIan A Wilson
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·S SteinbacherR Huber
Nov 26, 2010·The Journal of Antimicrobial Chemotherapy·Gallen B Triana-BaltzerFang Fang
May 27, 2011·Journal of Virology·Kazuya ShiratoFumihiro Taguchi
Jun 10, 2011·Journal of Virology·Mingyang WangGeorge F Gao
Dec 22, 2006·Journal of Virology·Eric LofgrenE N Naumova
Dec 21, 2007·Journal of Virology·Donna M TscherneCharles M Rice
Oct 28, 2004·Journal of Virology·Mikhail N MatrosovichHans-Dieter Klenk
Feb 26, 2005·Journal of Virology·Young-Min LeeCharles M Rice
Mar 8, 2008·Virology Journal·Karoline BragstadAnders Fomsgaard

❮ Previous
Next ❯