The neuritogenic and neuroprotective potential of senegenin against Aβ-induced neurotoxicity in PC 12 cells

BMC Complementary and Alternative Medicine
Robert Jesky, Hailong Chen

Abstract

Improved therapeutics aimed at ameliorating the devastating effects of neurodegenerative diseases, such as Alzheimer's disease (AD), are pertinent to help attenuate their growing prevalence worldwide. One promising avenue for such therapeutics lies in botanical medicines that have been efficaciously employed in the likes of traditional medicine doctrines for millennium. Integral to this approach is the necessity of neuritogenesis and/or neuroprotection to counterbalance the deleterious effects of amyloid-β (Aβ) proteins. Senegenin, a principle saponin of Polygala tenuifolia Willd., which has empirically shown to improve cognition and intelligence, was chosen to evaluate its cytoprotective potential and possible neuritogenic and neuroprotective effects. The purpose of the present study was then to analyze morphological changes in neurite development and altered protein expression of two proteins requisite to neuritogenesis, growth associated protein 43 (Gap-43) and microtubule-associated protein 2 (MAP2) in PC 12 cells. Neuritogenic analysis was conducted with immunofluorescence after incubation with Aβ (25-35) peptide, and to deduce information on cell viability and mitochondrial functionality MTT (3,(4,5-dimethylthiazol-2-yl)2...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Jan 1, 1990·Brain Research. Molecular Brain Research·B A YanknerR L Neve
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
Aug 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·K M YamadaN K Wessells
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·N W SeedsM W Nirenberg
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·M S ShearmanL L Iversen
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·K HensleyD A Butterfield
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M P LambertW L Klein
Nov 11, 1998·Nature Medicine·P S ErikssonF H Gage
Mar 17, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A N MurphyM F Beal
Apr 15, 1999·Annual Review of Neuroscience·A K McAllisterD C Lo
Mar 29, 2001·Physiological Reviews·D J Selkoe
Jun 12, 2001·Current Opinion in Neurobiology·A Patapoutian, L F Reichardt
Jun 18, 2002·Science·J Paul TaylorKenneth H Fischbeck
Oct 23, 2002·Journal of Neuroscience Research·Cheol Hyoung ParkYoo-Hun Suh
Oct 26, 2002·Science·Dennis J Selkoe
May 3, 2003·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·T YabeH Yamada
Mar 11, 2004·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Chihiro TohdaKatsuko Komatsu
Nov 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pascale N LacorWilliam L Klein
Dec 14, 2004·Cell Death and Differentiation·H KadowakiH Ichijo
Dec 16, 2004·Journal of Neural Transmission·M Nieto-Sampedro, M Nieto-Díaz
Feb 16, 2005·British Journal of Pharmacology·Tomoharu KuboyamaKatsuko Komatsu
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·William M Pardridge
Dec 20, 2005·Lancet·Cleusa P FerriUNKNOWN Alzheimer's Disease International
Dec 24, 2005·Nature Reviews. Neuroscience·Sara N Burke, Carol A Barnes
Feb 17, 2006·Neurobiology of Aging·Charles G Glabe
Mar 3, 2007·Biological & Pharmaceutical Bulletin·Fumito KarakidaMasaki Aburada
Apr 6, 2007·Biological & Pharmaceutical Bulletin·Ji Hyun KimInsop Shim
May 25, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ming-Jen HsuChien-Huang Lin

❮ Previous
Next ❯

Citations

Jan 31, 2017·International Journal of Molecular Sciences·Sravan Gopalkrishnashetty SreenivasmurthyMin Li
Jul 19, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pei-Pei Guan, Pu Wang
May 21, 2020·Pharmaceutical Biology·Xinxin DengChangcai Bai
Oct 6, 2020·Frontiers in Cell and Developmental Biology·Daayun ChungGabriela Caraveo
May 28, 2019·Current Medicinal Chemistry·Ze WangJing-Shan Shi
Jul 8, 2021·Molecular Biology Reports·Hamid Reza MoradiSajad Sahab Negah

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy

Related Concepts

Related Feeds

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.