The neuro-glial properties of adipose-derived adult stromal (ADAS) cells are not regulated by Notch 1 and are not derived from neural crest lineage

PloS One
Philip C WrageMalú G Tansey

Abstract

We investigated whether adipose-derived adult stromal (ADAS) are of neural crest origin and the extent to which Notch 1 regulates their growth and differentiation. Mouse ADAS cells cultured in media formulated for neural stem cells (NSC) displayed limited capacity for self-renewal, clonogenicity, and neurosphere formation compared to NSC from the subventricular zone in the hippocampus. Although ADAS cells expressed Nestin, GFAP, NSE and Tuj1 in vitro, exposure to NSC differentiation supplements did not induce mature neuronal marker expression. In contrast, in mesenchymal stem cell (MSC) media, ADAS cells retained their ability to proliferate and differentiate beyond 20 passages and expressed high levels of Nestin. In neuritizing cocktails, ADAS cells extended processes, downregulated Nestin expression, and displayed depolarization-induced Ca(2+) transients but no spontaneous or evoked neural network activity on Multi-Electrode Arrays. Deletion of Notch 1 in ADAS cell cultures grown in NSC proliferation medium did not significantly alter their proliferative potential in vitro or the differentiation-induced downregulation of Nestin. Co-culture of ADAS cells with fibroblasts that stably expressed the Notch ligand Jagged 1 or overe...Continue Reading

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Citations

Jan 15, 2014·Journal of Molecular Neuroscience : MN·Qiaoyu SunLili Zhang
Jan 29, 2009·Journal of Proteome Research·Raghothama ChaerkadyAkhilesh Pandey
Apr 6, 2013·Stem Cells and Development·Yuliang LiuRong Hou
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May 10, 2012·Cellular and Molecular Neurobiology·Chengcheng YingShiwen Li

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Methods Mentioned

BETA
transgenic
PCR
Fluorescence
transfection
FACS

Software Mentioned

Mesencult
Prism GraphPad
Mesenc
Amaxa
Pro Plus
GraphPad
Neurocult
Image
MetaMorph

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