The Neuro-spheroid--A novel 3D in vitro model for peripheral nerve regeneration

Journal of Neuroscience Methods
D KrausV Penna

Abstract

In order to reduce in vivo animal experiments in peripheral nerve regeneration research, in vitro models are desirable. Common two dimensional (2D) co-culture models lack the complex interactions of three dimensional (3D) physiological structures. The aim of the study was to establish a neuronal 3D spheroidal sprouting assay for peripheral nerve regeneration. Spheroids consisting of Schwann cells (SC, 500 cells/spheroid) and NG108-15 cells (NG, 50 cells/spheroid), a hybrid cell line, were formed in hanging drops and were embedded in a 3D collagen matrix. Spheroid sprout lengths were compared to those of the neurites of NG in a 2D co-culture with SC. Lengths were measured using phase contrast images taken every day over 10 days. Additionally we took fluorescence images to visualize the PKH26-labeled NG in both culture systems. Initially thin neurites grew out in both co-cultures, over time the sprouts' diameter in the 3D culture increased. The direct comparison of the sprout length revealed significantly longer neurites in the 3D co-culture from day 7 until day 10 (p<0.001). Other co-culture models either display processes in 2D or need complex matrices to create 3D structures. Our spheroidal model is easy to establish, highly f...Continue Reading

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Citations

Aug 27, 2015·The European Journal of Neuroscience·S GeunaC Grothe
May 14, 2016·Annals of Biomedical Engineering·Joseph VanderburghScott A Guelcher
Mar 9, 2018·MRS Communications·Radamés Ayala-CamineroJorge Almodovar
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Sep 18, 2018·Advanced Biosystems·Kristie M TevisMark W Grinstaff
Nov 28, 2018·Advanced Functional Materials·Jonathan M GrasmanDavid L Kaplan
Oct 20, 2021·ACS Applied Materials & Interfaces·Junjie ShenXianyou Zheng

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