PMID: 7513709May 1, 1994Paper

The neuronal chondroitin sulfate proteoglycan neurocan binds to the neural cell adhesion molecules Ng-CAM/L1/NILE and N-CAM, and inhibits neuronal adhesion and neurite outgrowth

The Journal of Cell Biology
D R FriedlanderM Grumet

Abstract

We have previously shown that aggregation of microbeads coated with N-CAM and Ng-CAM is inhibited by incubation with soluble neurocan, a chondroitin sulfate proteoglycan of brain, suggesting that neurocan binds to these cell adhesion molecules (Grumet, M., A. Flaccus, and R. U. Margolis. 1993. J. Cell Biol. 120:815). To investigate these interactions more directly, we have tested binding of soluble 125I-neurocan to microwells coated with different glycoproteins. Neurocan bound at high levels to Ng-CAM and N-CAM, but little or no binding was detected to myelin-associated glycoprotein, EGF receptor, fibronectin, laminin, and collagen IV. The binding to Ng-CAM and N-CAM was saturable and in each case Scatchard plots indicated a high affinity binding site with a dissociation constant of approximately 1 nM. Binding was significantly reduced after treatment of neurocan with chondroitinase, and free chondroitin sulfate inhibited binding of neurocan to Ng-CAM and N-CAM. These results indicate a role for chondroitin sulfate in this process, although the core glycoprotein also has binding activity. The COOH-terminal half of neurocan was shown to have binding properties essentially identical to those of the full-length proteoglycan. To st...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1992·Current Opinion in Cell Biology·T N WightE E Qwarnström
Oct 1, 1992·Current Opinion in Neurobiology·P Doherty, F S Walsh
Apr 1, 1992·Journal of Neurobiology·D M Snow, P C Letourneau
Nov 1, 1991·Journal of Neuroscience Research·J T PrinceW B Stallcup
Jan 1, 1991·Annual Review of Neuroscience·L F Reichardt, K J Tomaselli
Jan 1, 1991·Annual Review of Biochemistry·G M Edelman, K L Crossin
Dec 1, 1988·The Journal of Cell Biology·D R FriedlanderG M Edelman
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J W BeckerG M Edelman
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R BrackenburyG M Edelman
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M GrumetG M Edelman
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M GrumetG M Edelman
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Hoffman, G M Edelman
Jan 1, 1993·Annual Review of Neuroscience·M E SchwabC E Bandtlow
May 15, 1993·Experientia·R K Margolis, R U Margolis

❮ Previous
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Citations

Nov 15, 1996·Journal of Neuroscience Research·D K Shum, C H Chau
Mar 15, 1997·Journal of Neuroscience Research·A H ZischE B Pasquale
Feb 11, 1999·Journal of Neuroscience Research·G M Smith, M I Romero
Dec 3, 1999·Journal of Neuroscience Research·J LilienJ Balsamo
May 20, 1998·Molecular Neurobiology·K C BreenF D Hayes
Mar 1, 2012·Cell and Tissue Research·Hitoshi KawanoGeoffrey Raisman
Mar 1, 2012·Cell and Tissue Research·Michael K E Schäfer, Michael Frotscher
Jun 2, 2011·Cellular and Molecular Neurobiology·I NovotnaD Cizkova
Nov 26, 2010·Journal of Molecular Neuroscience : MN·Mir SajadHaider A Khan
Aug 28, 1995·Brain Research. Developmental Brain Research·K A Deyst, B P Toole
Jun 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D R CanningJ Silver
Jun 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J M SorreliA I Caplan
Oct 1, 1995·Current Opinion in Neurobiology·I AubertF H Gage
Mar 17, 2004·Molecular and Cellular Neurosciences·Tomoyuki MasudaTakashi Shiga
Feb 7, 1998·Journal of the Neurological Sciences·S M Chou, H S Wang
May 15, 2002·Brain Research. Molecular Brain Research·E M MuirJ H Rogers
Dec 13, 2001·Neurochemistry International·Galina A UshakovaGalina G Skibo
May 25, 1999·Progress in Neurobiology·R Probstmeier, P Pesheva
Mar 8, 2000·Progress in Neurobiology·P Bovolenta, I Fernaud-Espinosa
Sep 12, 2002·Biochimica Et Biophysica Acta·Takashi Angata, Els Brinkman-Van der Linden
Sep 14, 1999·Brain Research Bulletin·J W Fawcett, R A Asher
Oct 31, 1998·Gene·C K PrangeG G Lennon
Feb 1, 1996·Current Opinion in Neurobiology·H J Fryer, S Hockfield
Jun 12, 2003·Molecular and Cellular Neurosciences·Michael J JurynecCharles R Buck
Dec 31, 2003·Molecular and Cellular Neurosciences·Alexandre DobbertinAndreas Faissner
Sep 5, 2002·Progress in Retinal and Eye Research·Masaru Inatani, Hidenobu Tanihara

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