The neuronal noradrenaline transport system of PC-12 cells: kinetic analysis of the interaction between noradrenaline, Na+ and Cl- in transport

Naunyn-Schmiedeberg's Archives of Pharmacology
U Friedrich, H Bönisch

Abstract

The uptake of 3H-noradrenaline into reserpine-pretreated PC-12 cells (a clonal cell line which possesses "uptake1") was abolished when at high extracellular Cl- all the extracellular Na+ was replaced by Tris+ and when at high extracellular Na+ all the extracellular Cl- was replaced by isethionate. Increases in the external Cl- concentration (at a fixed high Na+ concentration) progressively increased the uptake of 3H-noradrenaline. The same was found with increase in the external Na+ concentration (at a fixed high Cl- concentration). From the anions tested only Br- and SCN- were able to partially mimic the transport-stimulating effect of Cl- (with about 40% and 20% effectiveness, respectively). When chloride was replaced by nitrate or larger anions such as sulphate, methylsulphate or isethionate, virtually no transport of 3H-noradrenaline was observed. The initial rate of uptake of 3H-noradrenaline showed saturation with increasing concentrations of noradrenaline when determined at several fixed concentrations of either Na+ or Cl-. The apparent Km for noradrenaline transport (KmNA) progressively decreased and the VmaxNA increased with increases in the concentration of Na+ (at a high concentration of Cl-) or Cl- (at a high concen...Continue Reading

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Citations

Aug 1, 1992·Naunyn-Schmiedeberg's Archives of Pharmacology·J Michael-HeppH Bönisch
May 1, 1996·Naunyn-Schmiedeberg's Archives of Pharmacology·A T CoreraJ J Bonnet
Dec 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·J LudwigU Trendelenburg
Dec 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·C J Zeitner, K H Graefe
Dec 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·H Bönisch, R Harder
Dec 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·E Schömig, H Bönisch
Sep 1, 1991·Trends in Pharmacological Sciences·U Trendelenburg
Jan 1, 1993·Brain Research Bulletin·D P FiglewiczI Ocrant
Nov 13, 2003·European Journal of Pharmacology·Margaret E Gnegy
Sep 12, 2002·European Journal of Pharmacology·Lana KantorMargaret Gnegy
Mar 12, 1998·Brain Research. Molecular Brain Research·M BrüssH Bönisch
Jun 18, 2002·Journal of Neurochemistry·Sonja SucicLesley J Bryan-Lluka
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·A GalliL J DeFelice
Sep 15, 1994·Annals of the New York Academy of Sciences·H Bönisch, M Brüss
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Feb 24, 2010·Neurochemistry International·Prashant MandelaGregory A Ordway
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Mar 27, 2015·Parkinson's Disease·Philippe HuotJonathan M Brotchie
Mar 11, 2005·The Journal of Biological Chemistry·Joel W SchwartzLouis J DeFelice
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Jan 1, 1989·Naunyn-Schmiedeberg's Archives of Pharmacology·A L UngellK H Graefe
Oct 4, 1993·Biochimica Et Biophysica Acta·G Rudnick, J Clark

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