The neuropeptide orexin A - search for its possible role in regulation of steroidogenesis in adult mice testes

Andrology
Deepanshu Joshi, Shio Kumar Singh

Abstract

Orexin A, a hypothalamic neuropeptide, regulates food intake and sleep-wake cycle by binding to orexin receptor 1. Besides brain, orexin A and orexin receptor 1 are also present in peripheral organs. In our earlier studies, localization and expression of orexin A and orexin receptor 1 have been shown in adult mouse testis, and further their role in testicular development in neonatal mouse was also demonstrated. In this study, role of orexin A and orexin receptor 1 in the testis of adult mouse by blocking binding of orexin A to orexin receptor 1 using an orexin receptor 1 antagonist, SB-334867, was investigated under in vivo and ex vivo conditions. Mice were given a single bilateral intratesticular injection of the antagonist at doses of 4 and 12 μg/mouse and were sacrificed 24 h post-injection. The antagonist treatment caused degenerative changes in the seminiferous tubules in the testis and also caused alterations in steroidogenesis, with a concomitant decrease in the level of testosterone (T) and an increase in the level of 17β-estradiol (E2 ) in serum and in testis. Further, expressions of SF1, StAR, P450scc and 17β-HSD were downregulated, while the expressions of 3β-HSD and P450arom were upregulated in antagonist-treated mi...Continue Reading

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Jul 16, 2020·Cell and Tissue Research·Carolina Felipe Alves de OliveiraGleide Fernandes de Avelar

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