The Neuroprotective Effects of Necrostatin-1 on Subarachnoid Hemorrhage in Rats Are Possibly Mediated by Preventing Blood-Brain Barrier Disruption and RIP3-Mediated Necroptosis

Cell Transplantation
Jing-Sen ChenGao Chen

Abstract

Despite the substantial efforts to elucidate the role of early brain injury in subarachnoid hemorrhage (SAH), an effective pharmaceutical therapy for patients with SAH continues to be unavailable. This study aims to reveal the role of necroptosis after SAH, and explore whether the disruption of the blood-brain barrier (BBB) and RIP3-mediated necroptosis following SAH in a rat SAH model are altered by necrostatin-1 via its selective inhibition of receptor-interacting protein kinase 1 (RIP1). Sixty-five rats were used in the experiments. The SAH model was established using endovascular perforation. Necrostatin-1 was intracerebroventricularly injected 1 h before SAH induction. The neuroprotective effects of necrostatin-1 were evaluated with multiple methods such as magnetic resonance imaging (MRI) scanning, immunohistochemistry, propidium iodide (PI) labeling, and western blotting. Pretreatment with necrostatin-1 attenuated brain swelling and reduced the lesion volume on T2 sequence and ventricular volume on MRI 72 h after SAH induction. Albumin leakage and the degradation of tight junction proteins were also ameliorated by necrostatin-1 administration. In addition, necrostatin-1 decreased the number of PI-positive cells in the ba...Continue Reading

References

Aug 1, 1969·The Journal of Speech and Hearing Disorders·M H Wessell
Apr 1, 1993·Journal of Neurosurgery·T MathiesenH von Holst
Jun 10, 1998·Neurological Research·P GaetaniF De Benedetti
Feb 7, 2001·Brain : a Journal of Neurology·J van Gijn, G J Rinkel
May 4, 2002·Stroke; a Journal of Cerebral Circulation·Jan ClaassenStephan A Mayer
Jan 13, 2006·Nature Chemical Biology·Alexei DegterevJunying Yuan
Feb 17, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Julian CahillJohn H Zhang
Jul 20, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yi YangGary A Rosenberg
Feb 3, 2007·Bioorganic & Medicinal Chemistry Letters·Ke WangChengye Yuan
Sep 18, 2007·Journal of Neuroscience Methods·Takashi SugawaraJohn H Zhang
Apr 15, 2008·Nature Chemical Biology·Alexei DegterevJunying Yuan
May 22, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Zerong YouMichael J Whalen
Jun 20, 2008·Journal of Neuroscience Methods·Ik-Seong ParkGavin W Britz
Sep 5, 2008·Bioorganic & Medicinal Chemistry Letters·Weihong ZhengChengye Yuan
Dec 10, 2008·Stroke; a Journal of Cerebral Circulation·Julian Cahill, John H Zhang
May 23, 2009·Stroke; a Journal of Cerebral Circulation·Takumi SozenJohn H Zhang
Jun 24, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Frances J NorthingtonLee J Martin
Dec 6, 2012·Stroke; a Journal of Cerebral Circulation·Shuichi OkuboGuohua Xi
Mar 12, 2013·Structure·Tian XieYigong Shi
Jul 31, 2013·Translational Stroke Research·Mutsumi FujiiJohn H Zhang
Oct 25, 2013·CNS & Neurological Disorders Drug Targets·S M FayazG K Rajanikant
Dec 11, 2013·The American Journal of Gastroenterology·Maria PierdomenicoSalvatore Cucchiara
Jan 31, 2014·The New England Journal of Medicine·Andreas Linkermann, Douglas R Green
Apr 15, 2014·International Journal of Cell Biology·Melanie D KingKrishnan M Dhandapani
Jun 7, 2014·Cell Death and Differentiation·W D CookD L Vaux
Dec 3, 2014·Molecular Cell·Pratyusha MandalWilliam J Kaiser
Apr 9, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Wei NiYa Hua
Sep 4, 2015·Neural Regeneration Research·Jing-Ru WuYan-Bo Cheng
Sep 17, 2015·Stroke; a Journal of Cerebral Circulation·Yusuke EgashiraGuohua Xi
Oct 12, 2015·International Immunopharmacology·Zhenyu JiaYang He
Dec 9, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Raul Chavez-ValdezFrances J Northington

❮ Previous
Next ❯

Citations

Apr 23, 2020·Frontiers in Cellular Neuroscience·Yuanjian FangJianmin Zhang
Nov 20, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sevda LuleMichael J Whalen
Nov 13, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Liyuan Cao, Wei Mu
Apr 18, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xu ZhaoHuijun Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
pharmacotherapies
dissection
protein assay
electrophoresis
X-ray
zymography

Software Mentioned

NIH ImageJ
SPSS
ImageJ
Prism
Quantity One

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier & Cytokines

Some cytokines are able to cross the blood brain barrier through transport systems and enter the cerebrospinal fluid and interstitial fluid spaces. Here is the latest research on cytokines crossing the blood brain barrier and how this can affect tissues within the CNS.